摘要
【目的】抗性淀粉(RS)和γ-氨基丁酸(GABA)是苦荞Fagopyrum tataricum功能性成分,对苦荞籽粒功能成分进行数量性状位点(QTL)定位对苦荞分子遗传改良具有重要意义。【方法】以‘小米荞’‘Xiaomiqiao’和‘晋荞麦2号’‘Jinqiaomai No.2’为亲本构建的重组自交系(XJ-RILs)群体作为供试材料,测定了XJ-RILs群体及其亲本的RS和GABA质量分数;基于遗传图谱定位控制RS和GABA的QTL,并对QTL区间内的候选基因进行鉴定和分析。【结果】XJ-RILs群体RS和GABA质量分数分别为4.51%~10.52%和0.37‰~2.50‰,表现出明显的超亲优势和偏正态分布。根据RS和GABA质量分数将XJ-RILs分为5类(C1~C5),其中C1类株系的RS和GABA质量分数均较高,从中筛选出高RS和高GABA优异株系各3株。对XJ-RILs群体的RS和GABA进行QTL定位,共检测到2个控制RS的QTL和1个控制GABA的QTL,这3个位点表型贡献率分别为7.59%、6.34%和5.05%。对QTL内的基因进行鉴定,共有108个RS候选基因,将其表达模式分为6类(R1~R6),其中R4、R5和R6中的30个基因在籽粒中的表达量较高。共鉴定到64个GABA候选基因,其表达模式可以分6类(G1~G6),其中G4、G5和G6中的21个基因在籽粒中的表达量较高。在RS和GABA候选基因中,分别有16和6个基因存在SNP/Indel变异。【结论】筛选出高RS和高GABA优异株系各3株,定位到2个控制RS和1个控制GABA的QTL位点,其区间内分别筛选到108和64个基因,初步锁定30个RS相关及21个GABA相关候选基因。
[Objective]Tartary buckwheat(Fagopyrum tataricum),as a high-quality minor grain crop,contains a variety of functional components,such as resistant starch(RS)andγ-aminobutyric acid(GABA).Quantitative trait locus(QTL)mapping of functional components in Tartary buckwheat grains is of great significance for molecular genetic improvement of Tartary buckwheat.[Method]The recombinant inbred lines population constructed by using‘Xiaomijiao’and‘Jinqiaomai No.2’as parents(XJ-RILs)was used as the test material.The contents of RS and GABA of the XJ-RILs population and its parents were determined.Based on the genetic map,QTLs controlling RS and GABA were located,and candidate genes within the QTL intervals were identified and analyzed.[Result]The XJ-RILs population exhibited significant transgressive segregation and a skewed normal distribution for RS and GABA contents,ranging from 4.51%to 10.52%and 0.37‰to 2.50‰,respectively.Based on their contents,the population was classified into five categories(C1 to C5),among which the C1 category had high levels of both RS and GABA,and three superior lines with high RS and three with high GABA were selected.QTL mapping for RS and GABA in the XJ-RILs population detected a total of two QTLs controlling RS and one controlling GABA,with phenotypic contribution rates of 7.59%,6.34%,and 5.05%,respectively.A total of 108 candidate genes for RS were identified within the QTL regions,and their expression patterns were classified into six categories(R1 to R6).Among them,30 genes in R4,R5,and R6 showed higher expression levels in seeds.Similarly,64 candidate genes for GABA were identified and their expression patterns were classified into six categories(G1 to G6),with 21 genes in G4,G5,and G6 exhibiting higher expression in seeds.Among the candidate genes for RS and GABA,16 and 6 genes,respectively,harbored SNP/Indel variations.[Conclusion]Three elite lines with high RS and three with high GABA were selected.Two QTL loci controlling RS and one QTL locus controlling GABA were identified.Within these loci intervals,108 and 64 genes were screened respectively,with 30 RS-related and 21 GABA-related candidate genes preliminarily identified.
作者
刘飞
黄娟
杜千禧
朱丽伟
邓娇
章洁琼
石桃雄
陈庆富
LIU Fei;HUANG Juan;DU Qianxi;ZHU Liwei;DENG Jiao;ZHANG Jieqiong;SHI Taoxiong;CHEN Qingfu(Research Center for Buckwheat Industry Technology,College of Life Sciences,Guizhou Normal University,Guiyang 550025,Guizhou,China;Guizhou Provincial Crop Technology Extension Station,Guiyang 550001,Guizhou,China)
出处
《浙江农林大学学报》
北大核心
2025年第4期703-713,共11页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(32060508)
贵州省科技计划项目(黔科合基础-ZK[2021]一般109)
国家现代农业产业技术体系荞麦育种岗位科学家专项资金(CARS-07-A5)
贵州师范大学学术新苗基金项目(黔师新苗[2021]A16号)。
