摘要
卡马西平(CBZ)是常用抗癫痫药,在水环境中频繁检出,其可以通过生物链进行累积,对生态系统具有潜在的威胁.本研究以莱茵衣藻和铜绿微囊藻为供试生物,分析了CBZ对真核与原核藻类的急性毒性效应.结果表明,以藻细胞生长和叶绿素a为毒性终点时,卡马西平对两种藻类均呈毒性作用,并且对莱茵衣藻的毒性作用均高于铜绿微囊藻.CBZ在暴露初期对莱茵衣藻的毒性较高,但随着时间推移而降低;而铜绿微囊藻则相反,从24~96 h,莱茵衣藻的EC_(50)逐渐由29.29 mg·L^(-1)升至120.60 mg·L^(-1);而铜绿微囊藻的EC50值则由861.3 mg·L^(-1)降至133.1 mg·L^(-1).CBZ能够提高藻细胞内ROS水平,导致氧化胁迫.相比于莱茵衣藻,铜绿微囊藻在CBZ暴露时表现出更强的药物降解能力、光合作用活性和抗氧化防御活性.莱茵衣藻胞内ROS远高于铜绿微囊藻,同时,莱茵衣藻的CAT和GSH等抗氧化防御系统相比于铜绿微囊藻受到更多的抑制,这使得莱茵衣藻受到更高的氧化胁迫和产生更多的MDA,导致CBZ对莱茵衣藻产生更强的毒性.
As a commonly used antiepileptic drug,carbamazepine(CBZ)has been frequently detected in surface water and it has been proven to be toxic to many aquatic organisms.CBZ can be enriched and accumulated through the food chain,posing a potential threat to the ecosystem.In this study,Chlamydomonas reinhardtii and Microcystis aeruginosa were used to illustrate toxicity of CBZ to eukaryotic and prokaryotic algae,respectively.The results showed that CBZ had acute toxicity to both algae with cell density and Chl-a content as end point.Meanwhile,the toxicity of CBZ to C.reinhardtii decreased with exposure time,whilst M.aeruginosa witnessed an increased toxicity of CBZ.CBZ could increase ROS level in both algae.Compared with M.aeruginosa,the ROS and MDA level was higher in C.reinhardtii and the activity of anti-oxidant system,including SOD,CAT and GSH,was lower.Our results also showed that the final concentration of CBZ after 96 h exposure in M.aeruginosa was lower than that in C.reinhardtii.And the lower CBZ concentration combined with higher anti-oxidant system activity might explain the more resistance of M.aeruginosa to CBZ than C.reinhardtii.
作者
陈亚松
房其昊
赵云鹏
周英萍
孙宛
肖琳
钱新
CHEN Yasong;FANG Qihao;ZHAO Yunpeng;ZHOU Yingping;SUN Wan;XIAO Lin;QIAN Xin(National Engineering Research Center of Eco-Environment in the Yangtze River Economic Belt,China Three Gorges,Wuhan 430010;State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023)
出处
《环境科学学报》
北大核心
2025年第7期433-440,共8页
Acta Scientiae Circumstantiae
基金
中国长江三峡集团公司科研项目(No.NBWL202300014)。
关键词
卡马西平
莱茵衣藻
铜绿微囊藻
氧化胁迫
毒性效应
carbamazepine
Chlamydomonas reinhardtii
Microcystis aeruginosa
oxidative stress
toxic effects
