摘要
Artificial conversion of CO_(2)into foods and chemicals offers a sustainable way to tackle population-relatedand environmental issues.Thermochemical and electrochemical methods for reducing CO_(2)to low-carbonmolecules have made significant progress;however,it remains challenging to generate long-chain,structurally diverse carbohydrates,which are the most abundant substances in nature.Here,we report thesuccessful design and realization of in vitro sucrose synthesis from C1 and C3 molecules through a thermodynamically favorable pathway with short reaction steps and low energy input.By engineering thephosphatase and sucrose synthase to improve their catalytic efficiency by 3-to 71-fold,and by optimizing the reaction system through an iterative scanning strategy,we achieved a high conversion yield of86%and a specific synthetic rate of 5.7 g L^(−1)h^(−1).Additionally,we demonstrated the cell-free starch synthesis without the need for dextrin primers,achieving a superior C1-to-starch synthesis rate compared topreviously developed multienzyme systems.Building upon this platform,we further extended methanolconversion to a variety of sugars,especially for cellooligosaccharides with a degree of polymerization ofCn≥18.Together,our system provides a promising,plant-independent route for de novo synthesis ofstructure-diversified oligosaccharides and polysaccharides.
基金
supported by the Strategic Priority ResearchProgram of the Chinese Academy of Sciences(XDC0120203)
the National Natural Science Foundation of China(32271545)
Major Program of Haihe Laboratory of Synthetic Biology(22HHSWSS00016 and 22HHSWSS00003)
Youth Promotion Association of Chinese Academy of Sciences(2021176),and TianjinYoung Scientific and Technological Talents(QN20230219).
