摘要
Although lettuce(Lactuca sativa L.)is known for its high flavonoid content,the regulatory mechanisms of flavonoid biosynthesis are yet to be fully investigated.A metabolic network profile was constructed based on metabolomic and transcriptomic datasets derived from five lettuce growth stages.From this analysis,we identified 201 metabolites and 15,775 co-expressed genes,which were subsequently organized into six distinct expression modules.A novel flavonoid biosynthesis regulatory network emerged,comprising the transcription factors Ls BBX20,Ls MYB114,and Ls MYB114-like.Each of these three factors was found to individually regulate the expression of flavonoid biosynthesis genes.Ls BBX20 further regulates the expression of Ls MYB114 and Ls MYB114-like in a light-dependent manner.The results of this study include not only a novel regulatory network of flavonoid biosynthesis but also a valuable dataset for the systematic investigation of metabolic regulation in lettuce.
基金
supported by the National Natural Science Foundation of China(32202471)
Natural Science Foundation of Sichuan Province,China(2023NSFSC1991,2023NSFSC0164)
the Local Financial Project of the National Agricultural Science and Technology Center(NASC2024TD03,NASC2023ST10)
the earmarked fund for Sichuan Innovation Team Program of CARS(sccxtd-2024-22)
the Agricultural Science and Technology Innovation Program of CAAS(ASTIP-IUA-2025002,S2024005)
the Fundamental Research Funds for the Central Universities(SCU2024D003)。
