摘要
试验旨在探究辽宁省猪源雷氏普罗威登斯菌的致病性与耐药性,无菌采集辽宁省营口市某养猪场发病仔猪的肝脏、肠道等病料,应用细菌分离培养和鉴定技术,结合分子生物学技术进行病原菌鉴定,并进行小鼠致病性试验、药敏试验。结果显示,试验分离到一株猪源雷氏普罗威登斯菌。分离株在10%绵羊鲜血营养琼脂培养基上形成灰白色、湿润凸起、中等大小菌落,且不溶血,革兰染色为两端钝圆的革兰氏阴性短杆菌。接种分离株可导致小鼠发病率为100%、死亡率为50%,并导致肝脏、肺脏、肠道等多器官发生病理损伤,证明猪源雷氏普罗威登斯菌对小鼠具有较强的致病性。分离株对头孢曲松、阿米卡星、环丙沙星、诺氟沙星、左氧氟沙星5种抗菌药物敏感,对头孢哌酮、庆大霉素中介,对氨苄西林、头孢唑林、头孢他啶等10种抗菌药物耐药。研究表明,猪源雷氏普罗威登斯菌辽宁株对多数抗菌药物已产生了耐药性,但仍有5种抗菌药物高度敏感,其中头孢曲松无兽用药剂,阿米卡星、诺氟沙星、左氧氟沙星属于兽用禁用药,只有环丙沙星可作为兽用药物用于本病防治。研究结果为猪源雷氏普罗威登斯菌病防控技术研究提供了一定参考。
This study aimed to investigate the pathogenicity and drug resistance of Providencia rettgeri isolated from pigs in Liaoning Province.Tissue samples(liver,intestine)were aseptically collected from diseased piglets on a farm in Yingkou City,Liaoning.Pathogen identification was performed using bacterial isolation/culture and molecular biology techniques,followed by mouse pathogenicity assays and antimicrobial susceptibility testing.Results showed that a strain of Providencia rettgeri was successfully isolated.Colonies appeared gray-white,moist,convex,and medium-sized on 10%sheep blood agar without hemolysis.Gram staining revealed Gram-negative short bacilli with rounded ends.Inoculation caused 100%morbidity and 50%mortality in mice,inducing pathological damage in multiple organs(liver,lungs,intestine),confirming strong pathogenicity.The isolate was sensitive to five antimicrobials:Ceftriaxone,amikacin,ciprofloxacin,norfloxacin,and levofloxacin,intermediate to cefoperazone and gentamicin,and resistant to ten others(including ampicillin,cefazolin,and ceftazidime).The study reveals that the Providencia rettgeri isolated from pigs in Liaoning Province has developed resistance to most antimicrobial agents.However,it remains highly susceptible to five antimicrobials:ceftriaxone(no approved veterinary formulation),amikacin,norfloxacin,and levofloxacin(internationally banned for veterinary use),leaving only ciprofloxacin as a clinically applicable veterinary drug for prevention and treatment.These findings provide valuable references for developing control strategies against Providencia rettgeri infections in swine.
作者
由莹
刘丽颖
周大勇
孙弋雅
任振堃
王辉暖
高慎阳
周铁忠
You Ying;Liu Liying;Zhou Dayong;Sun Yiya;Ren Zhenkun;Wang Huinuan;Gao Shenyang;Zhou Tiezhong(Liaoning Provincial Key Laboratory of Animal Product Quality and Safety,College of Animal Husbandry and Veterinary Medicine,Jinzhou Medical University,Liaoning Jinzhou 121001;Yingkou Animal Disease Prevention and Control Center,Liaoning Yingkou 115000;Beizhen Animal Disease Prevention Control Center,Liaoning Jinzhou 121300)
出处
《现代畜牧兽医》
2025年第7期11-16,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
辽宁省科学技术计划农业重大专项(2023JH11020006)。
关键词
猪
雷氏普罗威登斯菌
分离鉴定
致病性
耐药性
辽宁株
Swine
Providencia rettgeri
Isolation and identification
Pathogenicity
Drug resistance
Liaoning strain
