摘要
以紫色(Pu)、粉色(Pi)、白色(Wh)黄芩(Scutellaria baicalensis Georgi)花为试材,采用转录组测序及生物信息学分析方法,研究3个黄芩花转录因子表达特征,以期为揭示黄芩花青素的生物合成机制提供参考依据。结果表明:白花与紫花的差异性表达比较组中特异性表达基因有392个,其中上调102个,下调290个;其次为白花与粉花,特异性表达基因为121个,其中上调44个,下调77个;粉花与紫花的特异性表达基因最少为66个,其中上调31个,下调35个。使用K-means聚类方法分析不同样本间差异表达基因表达趋势,共聚类到15个集群,存在7个集群(2,4,7,10,12,13和15)在白花中的表达量低于粉花和紫花。使用GO富集分析不同聚类的生物学功能,Group 7主要聚类到脱落酸激活调节信号通路、花青素积累、多细胞组织发育等。3个比较组KEGG通路富集分析发现,白花与粉花比较组中差异表达基因主要富集在植物激素信号转导、植物中MAPK信号通路、黄酮和黄酮醇的生物合成。关键转录因子和花青素合成结构基因关联分析表明,bHLH、bZIP、WRKY和NAC与花青素合成结构基因显著相关。qRT-PCR分析表明,bHLH(Sbai7A132T37)、bZIP(Sbai3A7T134)、WRKY(Sbai1P459T36)、NAC(Sbai1P193T19)在白色花中表达量最高;WRKY(Sbai1C549T18)在粉色花中表达量最高。
Taking Scutellaria baicalensis Georgi flowers of purple(Pu),pink(Pi),and white(Wh)as experimental materials,and the transcriptional sequencing and bioinformatics analysis methods were applied to study the expression characteristics of three S.baicalensis Georgi flower transcription factors,in order to provide reference for laying a molecular foundation for revealing the biosynthesis mechanism of anthocyanins in S.baicalensis Georgi flowers.The results showed that there were 392 specific expressed genes in the comparison group of white flowers and purple flowers,including 102 up-regulated and 29 down-regulated;followed by white flowers and pink flowers,with 121 specific expressed genes,including 44 up-regulated and 77 down-regulated;least specific expressed genes were pink flowers and purple flowers,with 66,including 31 up-regulated and 35 down-regulated.The K-means clustering was used to analyze the trend of differentially expressed gene expression in different samples,and a total of 15 clusters were clustered,and the expression level of 7 clusters(2,4,7,10,12,13,and 15)in white flowers was lower than that in pink and purple flowers.The GO enrichment analysis was used to analyze the biological functions of different clusters,Group 7 was mainly clustered into abscisic acid activated regulatory signal pathway,anthocyanin accumulation and multi-cellular tissue development,etc.The KEGG pathway enrichment analysis of the three comparison groups showed that the differentially expressed genes in the comparison group of white flowers pink flowers were mainly enriched in plant hormone signal transduction,MAPK signaling pathway in plants,and the biosynthesis of flavonoids and flavonols in plants.The analysis between key transcription factors and anthocyanin synthesis structural genes showed that bHLH,bZIP,WRKY and NAC were significantly associated with the anthocyan synthesis structural genes.The qRT-PCR analysis showed that the expression levels of bHLH(Sbai7A132T37),bZIP(bai3A7T134),WRKY(Sbai1P459T36),and NAC(Sbai1P19319)were the highest in white flowers;WRKY(Sbai1C549T18)was the highest expressed in pink flowers.
作者
王敬
崔画
谢岩
高妍夏
郭新淼
孙志超
WANG Jing;CUI Hua;XIE Yan;GAO Yanxia;GUO Xinmiao;SUN Zhichao(Sericultural Research Institute,Chengde Medical University,Chengde,Hebei 067000;Department of Electronic and Communication Engineering,Chengde College of Applied Technology,Chengde,Hebei 067000)
出处
《北方园艺》
北大核心
2025年第15期12-20,共9页
Northern Horticulture
基金
河北省自然科学基金资助项目(H2023406059)
承德市基础研究专项资助项目(202205B069)
大学生创新创业训练计划资助项目(2024015)。
关键词
黄芩
花色
转录因子
花青素
Scutellaria baicalensis Georgi
flower color
transcription factors
anthocyanin
