摘要
目的了解北京市部分地区蜱种分布情况,以及携带病原体种类,为蜱传疾病的防控提供参考数据。方法2024年4—9月,在北京市6个郊区使用布旗法采集游离蜱虫标本;根据形态学鉴定及线粒体内细胞色素氧化酶亚基I(cytochrome C oxidase subunitⅠ,COI)基因和内转录间隔区2(internal transcribed spacer 2,ITS2)基因进行分子生物学鉴定,并使用MEGA 11.0软件构建进化树;采用多重荧光定量PCR法检测大别班达病毒、查菲埃立克体、伯氏疏螺旋体、斑点热群立克次体、巴尔通体、嗜吞噬细胞无形体、巴贝虫和泰勒虫8种蜱传病原体,并通过实时荧光定量PCR法检测荆门病毒。结果2024年在北京市共采集蜱虫标本1620只,分为168个蜱虫标本混合池,共获得129条COI基因序列和148条ITS2基因序列,两种基因鉴定结果一致的序列有118条。分子鉴定得到长角血蜱、日本血蜱和森林革蜱3种蜱种,检测到DBV、SFGR和嗜吞噬细胞无形体3种病原体,SFGR为劳氏立克次体。结论北京市的优势蜱种为长角血蜱,存在森林革蜱和日本血蜱,所携带的病原体以SFGR为主,其次为DBV,提示该地区应继续关注此病原体的监测和疾病的防控。
Objective To investigate the distribution of tick species and the associated pathogens in selected areas of Beijing,so as to provide reference data for the prevention and control of tick-borne diseases.MethodsFrom April to September 2024,free-living ticks from six suburban areas of Beijing were collected using the flagging method.The species were determined by morphological identification and molecular analyses targeting the mitochondrial cytochrome c oxidase subunit I(COI)gene and internal transcribed spacer 2(ITS2)gene.MEGA 11.0 was used to construct the phylogenetic tree.Eight tick-borne pathogens,including Dabie bandavirus(DBV),Ehrlichia chaffeensis,Borrelia burgdorferi,spotted fever group Rickettsia(SFGR),Bartonella spp.,Anaplasma phagocytophilum,Babesia spp.,and Theileria spp were screened via multiplex real-time qPCR.Jingmen virus was detected by real-time qPCR.ResultsA total of 1620 ticks were collected in 2024 and were divided into 168 pools.A total of 129 COI and 148 ITS2 sequences were obtained,with 118 sequences showing consistent result between both genes.Three tick species,Haemaphysalis longicornis,H.aponica,and Dermacentor silvarum were identified.Three pathogens,including DBV,SFGR and A.phagocytophilum were detected.ConclusionsThe dominant tick species in Beijing was H.longicornis,with co-circulation of D.silvarum and H.japonica.The pathogens carried by ticks were mainly SFGR,followed by DBV,indicating the need for continued surveillance and control measures against these pathogens in the region.
作者
张维嘉
李夫
窦相峰
金昊源
李丹
任振勇
李爽
陈艳伟
冯兆民
张代涛
孙玉兰
Zhang Weijia;Li Fu;Dou Xiangfeng;Jin Haoyuan;Li Dan;Ren Zhenyong;Li Shuang;Chen Yanwei;Feng Zhaomin;Zhang Daitao;Sun Yulan(Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China)
出处
《国际病毒学杂志》
北大核心
2025年第3期234-239,共6页
International Journal of Virology
基金
北京重大呼吸道传染病研究中心项目(BJRID2025-004)。
关键词
蜱
蜱种鉴定
进化分析
蜱媒病原体
Tick
Tick species identification
Phylogenetic analysis
Tick-borne pathogen
