摘要
Objective Atherosclerosis involves not only the narrowing of blood vessels and plaque accumulation but also changes in plaque composition and stability,all of which are critical for disease progression.Conventional imaging techniques such as magnetic resonance angiography(MRA)and digital subtraction angiography(DSA)primarily assess luminal narrowing and plaque size,but have limited capability in identifying plaque instability and inflammation within the vascular muscle wall.This study aimed to develop and evaluate a novel imaging approach using ligand-modified nanomagnetic contrast(lmNMC)nanoprobes in combination with molecular magnetic resonance imaging(mMRI)to visualize and quantify vascular inflammation and plaque characteristics in a rabbit model of atherosclerosis.Methods A rabbit model of atherosclerosis was established and underwent mMRI before and after administration of lmNMC nanoprobes.Radiomic features were extracted from segmented images using discrete wavelet transform(DWT)to assess spatial frequency changes and gray-level co-occurrence matrix(GLCM)analysis to evaluate textural properties.Further radiomic analysis was performed using neural network-based regression and clustering,including the application of self-organizing maps(SOMs)to validate the consistency of radiomic pattern between training and testing data.Results Radiomic analysis revealed significant changes in spatial frequency between pre-and post-contrast images in both the horizontal and vertical directions.GLCM analysis showed an increase in contrast from 0.08463 to 0.1021 and a slight decrease in homogeneity from 0.9593 to 0.9540.Energy values declined from 0.2256 to 0.2019,while correlation increased marginally from 0.9659 to 0.9708.Neural network regression demonstrated strong convergence between target and output coordinates.Additionally,SOM clustering revealed consistent weight locations and neighbor distances across datasets,supporting the reliability of the radiomic validation.Conclusion The integration of lmNMC nanoprobes with mMRI enables detailed visualization of atherosclerotic plaques and surrounding vascular inflammation in a preclinical model.This method shows promise for enhancing the characterization of unstable plaques and may facilitate early detection of high-risk atherosclerotic lesions,potentially improving diagnostic and therapeutic strategies.
基金
supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education(Grant number:RS-2023-00248763).
