摘要
目的:探讨聚苯乙烯微塑料(polystyrene microplastics,PS-MPs)联合高脂处理对血管内皮细胞的影响。方法:使用含5%胎牛血清的DMEM培养基培养人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)。对HUVECs采用常规培养、高脂处理和PS-MPs联合高脂处理。实验分为对照组、高脂处理组和PS-MPs联合高脂处理组。采用CCK-8试剂检测细胞活力,F-actin染色检测细胞形态变化情况,流式细胞术、划痕实验和管腔形成实验检测细胞凋亡、迁移和成管能力。结果:HUVECs暴露于高脂环境后,细胞活力明显降低,细胞形态皱缩,细胞凋亡水平明显增加,细胞迁移和成管能力均明显降低。相较于高脂处理组,PS-MPs联合高脂处理后,细胞活力,细胞形态,细胞凋亡水平和细胞迁移能力均没有明显变化,但细胞成管能力进一步受损。结论:高脂处理会影响细胞活力,改变细胞形态,损伤血管内皮细胞功能;在高脂处理基础上联合PS-MPs处理后,可加剧血管内皮细胞功能损伤。
Objective:To investigate the effect of polystyrene microplastics(PS-MPs)combined with high-fat treatment on vascular endothelial cells.Methods:Human umbilical vein endothelial cells(HUVECs)were cultured in the DMEM medium containing 5%fe-tal bovine serum.HUVECs were treated with conventional culture,high-fat treatment,and PS-MPs combined with high-fat treatment.The experiment was conducted in the three groups of control group,high-fat treatment group and PS-MPs+high-fat treatment group.CCK-8 assay was used to measure cell viability,F-actin staining was used to observe cell morphological changes,and flow cytometry,scratch assay,and tube formation assay were used to measure the apoptosis,migration,and tube-forming ability of cells.Results:After HUVECs were exposed to the high-fat environment,there was a significant reduction in cell viability,shrinkage of cells,a signifi-cant increase in cell apoptosis,and significant reductions in cell migration and tube-forming ability.Compared with the high-fat treat-ment group,there were no significant changes in cell viability,cell morphology,cell apoptosis,and cell migration ability after PS-MPs combined with high-fat treatment,but the tube-forming ability of cells was further impaired.Conclusion:High-fat treatment will affect cell viability,change cell morphology,and damage vascular endothelial cell function,and PS-MPs combined with high-fat treat-ment can aggravate the damage of vascular endothelial cell function.
作者
王静
刁家妮
龙菁
何渝广
谭丽萍
陈雪梅
李方方
何俊琳
王应雄
高茹菲
李伟轲
Wang Jing;Diao Jiani;Long Jing;He Yuguang;Tan Lipin;Chen Xuemei;Li Fangfang;He Junlin;Wang Yingxiong;Gao Rufei;Li Weike(Joint Laboratory of International Cooperation of the Ministry of Education for Reproduction and Development,School of Public Health,Chongqing Medical University)
出处
《重庆医科大学学报》
北大核心
2025年第7期969-976,共8页
Journal of Chongqing Medical University
基金
国家自然科学基金青年基金资助项目(编号:82301890)。
关键词
聚苯乙烯微塑料
高脂
人脐静脉血管内皮细胞
成管能力
polystyrene microplastics
high-fat
human umbilical vein endothelial cells
tube-forming ability
