摘要
目的齿槽嵴裂是唇腭裂患者中高发的先天性伴生畸形,会严重影响患者的正常牙齿萌出。目前临床上最常用的治疗方法是自体骨移植,但存在术后吸收率过高、难以进行牙齿种植、供体有限等问题。Bi基合金熔点比传统金属低,具有良好的可塑性和生物相容性,机械强度接近松质骨,可通过与温敏水凝胶协同注射的方式对骨缺损部位进行修复。本文采用Bi基合金-温敏水凝胶协同注射的方式修复兔齿槽嵴裂。方法首先通过体外3D打印兔齿槽嵴裂模型填充实验证明了Bi基合金-温敏水凝胶具有良好的可塑性,能够适应不规则的齿槽嵴裂形状。然后采用兔齿槽嵴裂动物模型,在兔牙槽骨上制备齿槽嵴裂,通过测算骨缺损体积,得到应注射的Bi基合金-温敏水凝胶的体积,随后在齿槽嵴裂处注射Bi基合金-温敏水凝胶。结果在齿槽嵴裂修复术后第0、1、2、4周,对齿槽嵴裂处进行CT扫描,结果显示修复效果良好,第4周时未出现移位或脱落。第4周处死实验兔后,将兔牙槽骨制成切片并进行HE染色,结果显示Bi基合金-温敏水凝胶与周围骨接触面处有胶原纤维产生。结论Bi基合金-温敏水凝胶能够适形化填充齿槽嵴裂,还能稳定存在于骨缺损处不会被自体吸收,在牙齿种植领域具有潜在的应用前景。
Objective Alveolar ridge cleft is a highly prevalent congenital deformity that often accompanies cleft lip and palate.It can seriously affect patients’normal tooth eruption.The most common clinical treatment currently is autologous bone grafting,but this has drawbacks such as a high postoperative resorption rate,difficulty in dental implantation and limited donor availability.Bi-based alloy has a lower melting point than traditional metals and offers good plasticity,biocompatibility and mechanical strength similar to cancellous bone.It can be co-injected with temperature-sensitive hydrogel to repair bone defects.In this study,we use a bismuth-based alloy and temperature-sensitive hydrogel co-injection technique to repair dentoalveolar ridge fractures in rabbits.Methods Firstly,the plasticity of the Bi-based alloy-temperature-sensitive hydrogel was proven,and its ability to adapt to the irregular shape of a dentoalveolar ridge fracture was demonstrated through filling experiments by using an in vitro 3D-printed rabbit dentoalveolar ridge fracture model.Then,an animal model of a rabbit alveolar ridge fracture was used to create a fracture on the alveolar bone of rabbits.The volume of the Bi-based alloy-temperature-sensitive hydrogel to be injected was determined by measuring the bone defect volume.The hydrogel was then injected into the fracture.Results CT scans were performed at weeks 0,1,2 and 4 after dentoalveolar ridge fracture repair at the fracture site.These scans showed that the repair was effective and that there was no displacement or detachment at week 4.After the rabbits were euthanised at week 4,sections of their alveolar bone were prepared and stained with haematoxylin and eosin(HE),revealing the production of collagen fibres at the interface between the Bi-based alloy-temperature-sensitive hydrogel and the surrounding bone.Conclusions The Bi-based thermosensitive hydrogel can fill alveolar ridge fractures in a conformal manner,existing stably in bone defects without being resorbed by the body.This has potential applications in dental implantation.
作者
刘思聪
姜婵媛
李雷
王乾宇
邓中山
LIU Sicong;JIANG Chanyuan;LI Lei;WANG Qianyu;DENG Zhongshan(Key Laboratory of Cryogenic Science and Technology,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190;Plastic Surgery Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100144;School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049)
出处
《北京生物医学工程》
2025年第4期340-346,共7页
Beijing Biomedical Engineering
关键词
齿槽嵴裂
Bi基合金
温敏水凝胶
牙槽骨植入
骨填充材料
alveolar ridge fracture
Bi-based alloy
temperature-sensitive hydrogel
alveolar bone implant
bone filling material
