摘要
TCP中的ClassⅡ转录因子亚家族对植物叶片、花发育及侧枝形成发挥核心调控作用,且可细分为CYC/TB1和CIN亚类。其中,CIN亚类在调控植物花器官发育中至关重要。为探究CIN亚类基因在蝴蝶兰(Phalaenopsis)花色及花发育中的功能,本研究分析了CIN亚类在蝴蝶兰不同器官的表达谱,确定PeCIN7为可能参与花发育及花色调控的候选基因。进化树分析显示,PeCIN7与拟南芥AtTCP3、AtTCP4同源性最高,拥有保守的bHLH结构;亚细胞定位结果表明,PeCIN7定位于细胞核。病毒诱导基因沉默试验表明,与对照组(Mock)相比,PeCIN7沉默株系的花色变浅,花型趋圆,长宽比显著下降。本研究结果为蝴蝶兰新品种开发提供了理论依据。
ClassⅡtranscription factor subfamily of TCP plays important roles in plant leaf and flower development as well as branch formation.This subfamily can be further divided into CYC/TB1 and CIN subclasses,with the CIN subclass being particularly crucial for regulating plant floral organ development.To investigate the function of these genes in the flower color and development of Phalaenopsis orchid,this study analyzed the expression profiling of CIN subclasses genes in different organs of Phalaenopsis orchid and PeCIN7 was identified as a candidate gene potentially regulating flower development and flower color.Phylogenetic analysis revealed that PeCIN7 was most closely related to AtTCP3 and AtTCP4 in Arabidopsis and had a conservative bHLH(basic helix-loop-helix)domain structure.Subcellular localization showed that PeCIN7 was localized in the nucleus.Virus-induced gene silencing(VIGS)experiments revealed that PeCIN7-silenced lines exhibited significantly lighter flower color,and had a near-circular morphology characterized by a markedly decreased length-width ratio.These findings provide a theoretical basis for the development of new Phalaenopsis varieties.
作者
郭鑫
徐信雅
李含霁
龙鑫
张英杰
孙纪霞
明凤
GUO Xin;XU Xinya;LI Hanji;LONG Xin;ZHANG Yingjie;SUN Jixia;MING Feng(College of Life Sciences,Shanghai Normal University,Shanghai 200234;Yantai Academy of Agricultural Sciences,Yantai,Shandong 265500)
出处
《核农学报》
北大核心
2025年第9期1886-1895,I0011,共11页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金面上项目(32472777)
上海师范大学本科生科研项目(24LKY073)。
关键词
转录因子PeCIN7
花发育
花色
蝴蝶兰
PeCIN7 transcription factors
flower development
flower color
Phalaenopsis orchid
