摘要
【目的】从艾(Artemisia argyi)全基因组数据中鉴定出BES1(BRI1-EMS-Suppressor1)基因家族成员,对其进行生物信息学分析,以阐明艾BES1基因家族的表达特性。【方法】使用在线分析软件对艾BES1家族成员的理化性质、启动子元件、系统进化关系和表达模式进行分析;利用转录组数据,明确各成员在艾不同组织的表达水平以及响应盐碱胁迫的表达情况;并采用实时荧光定量PCR(qRT-PCR)技术对AaBES1成员进行艾不同组织和应对植物生长调节剂处理的表达模式分析。【结果】从艾基因组中共鉴定获得14个AaBES1基因,AaBES1s蛋白亚细胞定位均在细胞核;AaBES1基因家族启动子含有多种逆境和激素响应元件;艾BES1家族成员响应盐碱与植物生长调节剂的胁迫,大多呈现先升高再下降的表达模式;与茎叶中表达相比,该家族成员在艾根中的表达模式具有组织特异性,表达量明显高于茎叶。【结论】通过对艾BES1基因家族进行鉴定和植物生长调节剂应答分析,表明艾BES1基因在盐碱胁迫和植物生长调节剂应答过程中发挥重要作用,并为进一步开展艾BES1基因功能的研究和植物抗逆性研究奠定理论基础。
【Objective】Members of BES1 family of Artemisia argyi were identified from the genome-wide data,and expressions analyzed by bioinformatic analysis.【Methods】Physicochemical properties,promoter elements,phylogenetic relationships,and expressions of the AaBES1s were determined using online software.Transcriptome data were used to obtain the expression of each member in response to saline-alkali stress.qRT-PCR was employed to detect the expressions of the genes in different tissues under various plant growth regulator treatments on A.argyi.【Results】Fourteen BES1s subcellularly localized in nucleus were identified from the A.argyi genome.The promoter contained a variety of adversity and hormone-responsive elements.The stress responses to salinity-alkali and hormones of the genes were tissue-specific with an expression pattern of initial increase followed by a decline.This gene family shows tissue-specific expression in roots with significantly higher abundance compared to stems and leaves.【Conclusion】The AaBES1 family played an important role in the process of A.argyi in response to the stresses of salinity-alkali and plant growth regulator.
作者
李晶晶
刘秀玉
陈随清
王利丽
郭晨宁
马蕊
练从龙
LI Jingjing;LIU Xiuyu;CHEN Suiqing;WANG Lili;GUO Chenning;MA Rui;LIAN Congong(College of Pharmacy,Henan University of Chinese Medicine/Henan Key Laboratory of Chinese Medicine Resources and Chemistry/Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao,Zhengzhou,Henan 450046,China)
出处
《福建农业学报》
北大核心
2025年第5期476-486,共11页
Fujian Journal of Agricultural Sciences
基金
国家自然科学基金项目(82204551)
中央本级重大增减支项目(2060302)
河南省高等学校重点科研项目(22A360012)。
