摘要
针对市面上含有人参成分的进口乳液、膏霜和面膜3种化妆品基质中6种人参皂苷(Rb1、Rb2、Rc、Rd、Re和Rg1)成分测定建立快速高效的前处理方法,应用高效液相色谱-串联质谱仪,对进口人参类化妆品中6种人参皂苷成分进行定性和定量分析。建立的测定方法主要在色谱和质谱条件方面进行了优化,提高了检测灵敏度和准确性。样品经乙醇水超声提取,提取液用C18固相萃取柱净化,氮吹复溶后,乙腈-0.1%甲酸水为流动相,应用高效液相色谱-串联质谱仪进行检测,多反应检测模式,负离子模式扫描。经验证,6种人参皂苷在0.2~100μg/L浓度范围内线性关系良好(r>0.9995),方法检出限为0.01~0.06μg/kg,定量限为0.02~0.5μg/kg,满足测定需求。对33种空白样品分别加3种浓度(5、10、20μg/kg,n=6),检测结果相对标准偏差均小于5%,且加标回收率均大于80%。用该方法对进口人参类化妆品中的6种人参皂苷进行定性定量分析,灵敏度高、重现性好,且具有较高的精密度和准确度。可为海关对进口人参类化妆品的监管提供评估数据和技术支持。
A rapid and efficient pre-treatment was established for determining six ginsenosides(Rb1,Rb2,Rc,Rd,Re and Rg1)in three types of ginseng-containing imported available on the market:emulsions,creams,and facial masks.Qualitative and quantitative analyses of these ginsenosides was carried out using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The developed method was optimized mainly in terms of chromatographic and mass spectrometric conditions to improve the detection sensitivity and accuracy.Samples were extracted by ultrasonic ethanol-water method.The extracted was purified by C18 solid-phase extraction column and reconstituted after nitrogen evaporation.Detection was performed using HPLC-MS/MS with acetonitrile-0.1%formic acid aqueous solution as the mobile phase,under multiple reaction monitoring(MRM)mode and negative ion scanning.After method verification,the six ginsenosides showed good linearity in the concentration range of 0.2~100μg/L(r>0.9995).The detection limit of the method was 0.01~0.06μg/kg,and the quantification limit was 0.02~0.5μg/kg,which meets the measurement requirements.Thirty-three blank samples were each spiked at three concentration levels(5,10,20μg/kg,n=6).The relative standard deviations were all below 5%and the spiked recovery rates were all above 80%.This method is used for qualitative and quantitative analysis of six ginsenosides in imported ginseng cosmetics,which offers high sensitivity,good repeatability,high accuracy,and precision.This method can provide assessment data and technical support for the customs supervision for imported ginseng cosmetics.
作者
赵韫慧
王嘉怡
于淼淼
翟亚楠
杨璐
迟迅
ZHAO Yun-hui;WANG Jia-yi;YU Miao-miao;ZHAI Ya-nan;YANG Lu;CHI Xun(Changchun Customs Technology Center,Changchun 130062,China;Innovation Practice Center,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《化学试剂》
2025年第8期81-86,共6页
Chemical Reagents
基金
吉林省中医药科技项目(2023080)
海科中心自立科研课题项目(2024HX04)。
关键词
人参类化妆品
皂苷
高效液相色谱-串联质谱
定性
定量
固相萃取柱
ginseng-based cosmetics
ginsenoside
high performance liquid chromatography tandem mass spectrometry
qualitative analysis
quantitative analysis
solid-phase extraction columns
