摘要
【目的】构建基于SRAP分子标记技术的石斛兰杂交后代真实性鉴定方法,为石斛兰品种(系)鉴定提供可靠技术支撑。【方法】利用SRAP分子标记技术,对报春石斛、檀香石斛以及以报春石斛为母本、檀香石斛为父本的151个杂交后代进行真实性鉴定及遗传多样性分析。【结果】从90对SRAP引物中筛选7对特异性引物,实现对杂交后代的高效鉴定,效率达100%;多态性分析显示,7对引物的多态性比例为81.03%,揭示了丰富的遗传变异。聚类分析表明,在遗传距离为0.24时,153个样本材料可分为五个类群;82.78%的杂交后代与父本聚为一类,亲缘关系更近;杂交后代株系间的遗传相似系数普遍高于其与双亲的遗传相似系数,父本和母本与杂交后代株系的遗传相似系数接近。【结论】SRAP分子标记可用于石斛兰杂交后代早期鉴定,能够有效辅助石斛兰新品种鉴定和选育。
【Objective】The authenticity identification method of Dendrobium hybrid progeny based on SRAP marker technology was constructed to provide reliable technical support for the identification of new varieties(lines)of Dendrobium.【Method】Using SRAP marker technology,the authenticity identification and genetic diversity analysis of D.primulinum,D.anosmum and 151 hybrid progeny which were from D.primulinum as female parent and D.anosmum as male parent were carried out.【Result】7 pairs of specific primers were screened from 90 pairs of SRAP primers to achieve efficient identification for hybrid progeny,with an efficiency of 100%;Polymorphism analysis showed that the polymorphism ratio of 7 pairs of primers was 81.03%,revealing rich genetic variation;Cluster analysis showed that 153 sample materials could be divided into five groups when the genetic distance was 0.24.Among them,82.78%of the hybrid progeny were clustered with male parent,and the genetic relationship was closer.The genetic similarity coefficient among the hybrid progeny lines was generally higher than that between them and the two parents,and the genetic similarity coefficient between the male parent and the hybrid progeny lines was close to that between the female parent and the hybrid progeny lines.【Conclusion】SRAP marker can be used for the early identification of the hybrid progeny of Dendrobium,which can effectively assist the identification and breeding of new varieties.
作者
林榕燕
陈艺荃
林觅
伍莉娟
钟海丰
Lin Rongyan;Chen Yiquan;Lin Mi;Wu Lijuan;Zhong Haifeng(Crop Research Institute,Fujian Academy of Agricultural Sciences(Fujian Germplasm Resource Center),Fuzhou,Fujian 350013,China;Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou,Fujian 350013,China;Qingliu County Economic Crop Technology Promotion Station,Sanming,Fujian 365399,China)
出处
《东南园艺》
2025年第2期162-168,共7页
Southeast Horticulture
基金
福建省人民政府与中国农业科学院农业高质量发展超越‘5511’协同创新工程项目(XTCXGC2021016)
福建省属公益类科研院所基本科研专项(2022R1031002、2022R1031009)
福建省农业科学院自由探索科技创新项目(ZYTS2023003)
福建省农业科学院科技创新团队建设项目(CXTD2021010-2)。
关键词
石斛兰
SRAP标记
多态性
真实性鉴定
遗传多样性
特异性引物
Dendrobium
SRAP marker technology
polymorphism
authenticity identification
genetic diversity
specific primer
