摘要
本文重点论述了传统酶固定化方法(吸附法、交联法、包埋法、共价结合法)中载体材料的创新及其应用特点,以及新型固定化策略(复合固定化、共固定化、原位固定化、智能响应性固定化、生物正交固定化、纳米材料固定化法)的作用机制与性能优势。探讨了固定化酶在食品加工、医药研发和生物催化等领域的应用基础研究成果及产业化潜力。针对固定化过程中的酶活损失和底物传质阻力等问题,提出了优化方向,为固定化酶技术的工业化应用提供理论参考。
This review summarizes recent advances in enzyme immobilization technology.It focuses on carrier material innovations and applications in traditional immobilization methods(adsorption,cross-linking,entrapment,and covalent binding),as well as the mechanisms and advantages of novel immobilization strategies(composite immobilization,co-immobilization,in-situ immobilization,smart responsive immobilization,bioorthogonal immobilization,and nanomaterial immobilization).It also discusses the basic research achievements and industrialization potential of immobilized enzymes in fields such as food processing,pharmaceutical research and development,and biocatalysis.Optimization directions are proposed to address challenges such as enzyme activity loss and substrate mass transfer resistance,providing theoretical reference for industrial applications of immobilized enzymetechnology.
作者
严昕怡
大村拓
朱江煜
Yan Xinyi;Omura Taku;Zhu Jiangyu(School of Food Science and Engineering,Yangzhou University,Yangzhou,225000,China;Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,113-8657,Japan)
基金
国家自然科学基金项目(22208285)
中国博士后科学基金项目(2024M762732)资助。
关键词
酶固定化技术
固定化载体
固定化方法
生物催化
Enzyme immobilization technology
Immobilization carriers
Immobilization methods
Biocatalysis
