摘要
[目的]为了解山东省聊城市奶山羊乳腺炎金黄色葡萄球菌(S.aureus)流行现状及其耐药基因分布情况。[方法]采集山东省聊城市部分奶山羊场178份乳样,通过细菌培养、血浆凝固酶试验和PCR生物学技术进行分离鉴定S.aureus,分析分离菌株耐药性。[结果]鉴定出23株S.aureus,检出率为12.92%;分离菌株对青霉素、强力霉素、链霉素、克拉霉素和四环素耐药性较强;耐药基因PCR检测发现,10种耐药基因均有检出。其中,β-内酰胺类耐药基因mecA,大环内酯类耐药基因ermA、ermB、ermC,四环素类耐药基因tet,氨基糖苷类耐药基因aac(6')-Ⅰa、aph(2'')-Ⅰb、ant(4',4'')、aph(3')-Ⅱ,磺胺类耐药基因sul1平均携带率分别为30.43%、31.88%、43.48%、21.73%、8.70%,所有菌株至少携带2种耐药基因,最多7种耐药基因,以携带4~5种耐药基因菌株数量最多。[结论]山东省聊城市奶山羊场存在S.aureus流行传播风险,且菌株表现不同程度的耐药性,耐药基因携带率较高,应引起重视,加强监测和防控。
Common abomasum diseases in dairy cows include abomasum food accumulation,abomasum gastritis,abomasum displacement,etc.The loss of appetite and rumination of sick dairy cows cause abomasum,epigastrium secret knot and rumen gas,which seriously affects production performance and service life.In order to improve the prevention and treatment effect of common abomasum diseases in dairy cows,this paper summarized the causes,clinical symptoms,diagnosis and treatment methods of these diseases,in order to help the healthy breeding of dairy cows in pasture.
作者
亓爱杰
李莹
李路胜
QI Aijie;LI Ying;LI Lusheng(Yanggu County Animal Husbandry and Veterinary Development Center,Yanggu Shandong 252300;Liaocheng City Animal Husbandry and Veterinary Development Center,Liaocheng Shandong 252800;Agricultural College of Liaocheng University,Liaocheng Shandong 252800)
出处
《中国乳业》
2025年第7期71-76,83,共7页
China Dairy
基金
2023年山东省重点研发计划(农业良种工程品种培育)项目-奶牛关键性状智能选育技术研究与应用(2023LZGC004)。
