摘要
【目的】明确不同马铃薯品种对疮痂病的抗性与薯皮及薯肉颜色的相关性,探讨花青素含量、皮孔密度、薯皮厚度、薯皮结构等因素在马铃薯抗疮痂病中的作用。【方法】选取15个具有不同薯皮或薯肉颜色的马铃薯品种进行盆栽种植,接种致病链霉菌(Streptomyces.acidiscabies)后,采用扫描电镜观察薯皮结构并测定薯皮厚度,利用亚加蓝染色法测定皮孔数,分光光度计测定花青素和木质素含量,滴定法测定钙含量,分析薯块表型及抗性相关物质与抗性的关系。【结果】不同薯皮和薯肉颜色的马铃薯品种对疮痂病的抗性依次是红色、紫色、黄色、白色,其中红玫瑰3号、剑川红和丽薯14号品种的抗性最强,云薯303、丽薯6号、滇薯6号抗性最弱。薯皮和薯肉平均花青素含量由高到低依次是紫色、红色、黄色、白色。抗病品种薯皮更厚,单位面积皮孔数更少,钙含量、木质素含量更高;在未接种的马铃薯中,黑玫瑰L8号的薯皮最厚且细胞层数多,排列整齐致密;丽薯14号皮孔密度最小;剑川红、丽薯14号的钙含量最高,师薯6号的木质素含量最高。此外,薯皮花青素、薯肉花青素、薯皮厚度、钙含量、木质素与疮痂指数呈负相关关系,而皮孔数与疮痂指数呈显著正相关关系。【结论】马铃薯块茎的皮孔密度越小、薯皮越厚、钙含量和木质素含量越高,其对疮痂病的抗性越强;在薯皮厚度无明显差异的情况下,周皮细胞层数多、排列紧密整齐且细胞大小均匀的马铃薯品种表现出更强的抗病性。薯皮和薯肉颜色较深或者花青素含量较高的马铃薯品种对疮痂病的抗性更强。
[Objective]The study investigated the correlation between potato varieties'resistance to common scab and their skin and flesh colors.Additionally,it explored the roles of anthocyanin content,pore density,skin thickness,and skin structure in conferring disease resistance.[Method]Fifteen potato varieties with distinct skin and flesh colors were inoculated with Streptomyces acidiscabies in pots.Scanning electron microscopy(SEM)was used to examine skin structure and measure skin thickness,methylene blue staining determined pore density.Anthocyanin and lignin contents were quantified using spectrophotometry,and calcium content was measured by titration method.The relationships between tuber phenotype,resistance-related compounds,and disease resistance were analyzed.[Result]Potato varieties with red skin or flesh exhibited the highest resistance to common scab,followed by purple,yellow and white varieties.Among the varieties tested,Hongmei 3,Jianchuan Red,and Lishuo 14 showed the strongest resistance,while Yunshuo 303,Lishuo 6,and Dianshu 6 were the most susceptible.Purple varieties had the highest average anthocyanin content,followed by red,yellow and white varieties.Disease-resistant varieties had thicker skin,lower pore density,and higher calcium and lignin contents.In uninoculated samples,Heimei L8 exhibited the thickest skin,with multiple cell layers and a dense arrangement,and Lishuo 14 had the lowest pore density.Jianchuan Red and Lishuo 14 showed the highest calcium content;and Shishu 6 had the highest lignin content.Furthermore,anthocyanin content,skin thickness,calcium content,and lignin levels were negatively correlated with the scab index,while pore density was positively correlated.[Conclusion]Potato tubers with lower pore density,thicker skin,higher calcium and lignin contents exhibit greater resistance to common scab.When skin thickness is similar,varieties with more layers tightly arranges,and uniformly sized periderm cells demonstrate superior disease resistance.Additionally,varieties with darker skin and flesh colors or higher anthocyanin content display stronger resistance to common scab.
作者
徐亚锦
陈梦
刘春江
张越
杨艳丽
赵婧
刘霞
XU Ya-jin;CHEN Meng;LIU Chun-jiang;ZHANG Yue;YANG Yan-li;ZHAO Jing;LIU Xia(College of Plant Protection,Yunnan Agricultural University/Yunnan Provincial Key Laboratory of Plant Pathology,Kunming 650201,China)
出处
《西南农业学报》
北大核心
2025年第5期1060-1068,共9页
Southwest China Journal of Agricultural Sciences
基金
云南省重大科技专项(202402AE090017)
云南省农业基础研究联合专项(202301BD070001-063)
云南省现代农业马铃薯产业技术体系(2024KJTX-04)。
关键词
马铃薯
疮痂病
薯皮颜色
薯肉颜色
抗性机制
Potato
Common scab
Skin color
Flesh color
Resistance mechanisms
