摘要
【目的】探索青枯病菌(Ralstonia solanacearum)胁迫下马铃薯根际分泌物种类和代谢途径的变化及其影响机制,明确根系分泌物与病原微生物,以及由根系分泌物介导的植物-病原微生物-微生态之间的关系。【方法】基于LC-MS技术,分析青枯病原菌胁迫对马铃薯根系分泌物组成的影响,通过PCA、PLS-DA、单因素方差分析多元统计方法,探究根系分泌物的差异及代谢通路。【结果】马铃薯根系分泌物在接种青枯病菌3 d后成分差异随侵染时间增加而逐渐增大,共鉴定差异代谢产物10类1619种;随接种时间增加,糖类、核酸类、次级代谢因子等38种化合物也随之增加;而抗生素类、酰胺类等12种化合物含量随青枯病菌接种时间的延长而减少;青枯病菌接种对ABC转运蛋白代谢、赖氨酸降解、色氨酸代谢这3种代谢通路的影响严重。【结论】青枯病菌侵染马铃薯后,随着侵染时间增加,马铃薯根系分泌物成分差异逐渐增大,差异代谢物种类也随之增多。青枯病菌侵染通过降低ABC转运蛋白通路下化合物合成影响其蛋白合成,促进赖氨酸降解,调节吲哚乙酸合成,从植物生长调控、抗逆性改变及次生代谢物合成等方面影响马铃薯抗病能力。
[Objective]The study aimed to explore the types,metabolic pathways,and influencing mechanisms of potato rhizosphere secretions under Ralstonia solanacearum stress,to clarify the relationship between root exudates and pathogenic microorganisms,as well as the plant pathogen microecology mediated by root exudates.[Method]The impact of bacterial wilt stress on the composition of potato root exudates using LC-MS technology was analyzed.Through PCA,PLS-DA,and one-way ANOVA multivariate statistical methods,the dfferences of root exudates and metabolic pathways among different groups were studied.[Result]After 3 days of inoculation with R.solanacearum,the differences in the composition of potato root exudates increased gradually with the increase of infection time,and a total of 1619 different metabolites were identified in 10 categories;As the inoculation time increased,the content of 38 compounds such as sugars,nucleic acids,and secondary metabolic factors gradually increased,while the content of 12 compounds such as antibiotics and amides decreased with the inoculation of P.aeruginosa;The inoculation of P.aeruginosa had a significant impact on the metabolism of ABC transporters,lysine degradation,and tryptophan metabolism.[Conclusion]After infection by R.solanacearum,the following patterns are observed in potatoes.With the increase of infection time,the differences in the components of potato root exudates gradually increase,and the types of differential metabolites also increase accordingly.The infection of R.solanacearum affects the synthesis of ABC transporter proteins by reducing the synthesis of compounds in the ABC transporter pathway,promote the degradation of lysine,regulates the synthesis of indole-3-acetic acid,and influences the disease resistance of potatoes through aspects such as plant growth regulation,alteration of stress resistance,and synthesis of secondary metabolites.
作者
张庆
杨济达
杨艳丽
伏成秀
朱红业
邓思合
余顺平
赵定云
ZHANG Qing;YANG Ji-da;YANG Yan-li;FU Cheng-xiu;ZHU Hong-ye;DENG Si-he;YU Shun-ping;ZHAO Ding-yun(Yunnan Agricultural University,Kunming 650201,China;Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Weishan County Bureau of Agriculture and Rural Affairs,Dali,Yunnan 672499,China)
出处
《西南农业学报》
北大核心
2025年第5期1047-1059,共13页
Southwest China Journal of Agricultural Sciences
基金
云南省创新引导与科技型企业培育计划(202204BI090024)
云南省基础研究专项青年项目(202201AU070078)
云南实验室建设专项(202305AR340003-05)。
关键词
马铃薯
青枯病菌
非靶向代谢组
根系分泌物
响应
Potato
Ralstonia solanacearum
Non-targeted metabolomics
Rot exudates
Response
