摘要
目的对山东东营某育肥羊场发病绵羊的病原菌进行分离鉴定和提供治疗方案。方法采集病死羊肝脏组织,通过细菌分离、革兰氏染色、药敏试验、小鼠敏感性试验、细菌生化试验以及全基因组测序对分离的菌株进行鉴定和分析。结果分离的菌株(命名为SdDy)为革兰氏阳性链球菌,经全基因组测序和生物信息学分析确定为马肠链球菌。该菌株主要参与到代谢过程,对昆明白小鼠具有一定的致病性,对青霉素、氨苄西林、头孢噻呋钠、头孢曲松钠等4种抗生素敏感,对链霉素、阿莫西林、四环素、庆大霉素等4种抗生素耐药,经头孢噻呋钠对发病羊群进行针对性治疗,病情在2 d后得到有效控制。结论本研究成功分离了一株羊源马肠链球菌,并利用全基因组测序技术对其基因组特征进行分析,为羊源马肠链球菌生物学特性、公共卫生及疾病防治与流行病学调查提供参考资料。
Streptococcus equinus is a zoonotic disease that can cause illness in various animals under specific environmental conditions.No reports have described isolation of this bacterium from the liver in affected sheep.This study successfully isolated and identified a strain of Streptococcus equinus through bacterial isolation and culture,Gram staining,drug sensitivity testing,mouse sensitivity testing,bacterial biochemical testing,and whole genome sequencing.The strain was found to have pathogenicity toward Kunming white mice,and to be sensitive to four antibiotics(penicillin,ampicillin,ceftiofur sodium,and ceftriaxone sodium)but resistant to four antibiotics(streptomycin,amoxicillin,tetracycline,and gentamicin).On the basis of drug sensitivity testing,targeted treatment of the affected sheep flock with ceftiofur sodium effectively controlled the disease within 2 days,and no new cases occurred.This study provides a reference for biological characterization of ovine Streptococcus equinus;public health;and the investigation of disease prevention,control,and epidemiology.
作者
王海丽
尹秀升
贾琪
张峰源
莫玲
李林
董婧
王金良
WANG Hai-li;YIN Xiu-sheng;JIA Qi;ZHANG Feng-yuan;MO Ling;LI Lin;DONG Jing;WANG Jin-liang(Liaocheng Vocational&Technical College,Liaocheng 252000,China;Shandong Binzhou Animal Science and Veterinary Medicine Academy,Binzhou 256600,China;Binzhou Center for Disease Control and Prevention,Binzhou 256600,China;College of Life Science and Food Engineering,Hebei University of Engineering,Handan 056038,China;College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang 110866,China)
出处
《中国人兽共患病学报》
北大核心
2025年第6期641-647,共7页
Chinese Journal of Zoonoses
基金
山东省羊产业技术体系岗位专家资助项目(No.SDAIT-10-06)
科技创新2030农业生物育种重大项目(No.2022ZD04013)。
关键词
绵羊
马肠链球菌
分离鉴定
药敏试验
基因组分析
sheep
Streptococcus equinus
isolation and identification
drug susceptibility testing
genome analysis
