摘要
生物土壤结皮(Biological Soil Crusts)是荒漠生态系统的关键组成,其中蕴含的丰富多样的碳降解菌株能够将有机碳转化为无机碳形式,在促进有机质分解和维持荒漠生态系统碳循环过程中发挥着重要作用。本研究选取沙坡头固沙植被区的生物土壤结皮为研究对象,旨在筛选并鉴定具有不同碳源类型降解能力的功能菌株,并评估其酶活性。结果显示:菌株D7、D8的淀粉酶活性最高,分别为1.52、1.69 U·mL^(-1);G5菌株的果胶酶活性最高,为1.01 U·mL^(-1);菌株X2、X5的纤维素酶活性最高,分别为1.59、2.19 U·mL^(-1);通过苯胺蓝脱色法测定,M1菌株显示出较强的木质素过氧化物酶活性。经过分子生物学鉴定,这些菌株主要包括Pantoea alhagi(D7、D8、X5)、Bacillus subtilis(X2)、Erwinia piriflorinigrans(G5)和Cupriavidus respiracul(M1)。
Biological soil crusts(BSCs)are integral to desert ecosystems,facilitating the conversion of organic carbon to inorganic forms through a diverse array of carbon-degrading strains,thereby promoting the decomposition of organic matter and the carbon cycle in desert ecosystems.This study focused on BSCs from the Shapotou sand-fixing vegetation area,aiming to screen and identify functional strains with the ability to degrade different types of carbon sources and to evaluate their enzyme activities.The results showed that strains D7 and D8 had the highest amylase activity,at 1.52 U·mL^(-1)and 1.69 U·mL^(-1),respectively;strain G5 had the highest pectinase activity,at 1.01 U·mL^(-1);strains X2 and X5 had the highest cellulase activity,at 1.59 U·mL^(-1)and 2.19 U.mL,respectively.Strain Ml displayed significant lignin peroxidase activity as determined by the aniline blue decolorization assay.Molecular biological identification revealed that these strains primarily belong to Pantoea alhagi(D7、D8、X5),Bacillus subtilis(X2),Erwinia piriflorinigrans(G5),and Cupriavidus respiraculi(M1).
作者
赵丽娜
谢燚谛
贺子康
柴梦洋
高源婧坤
吴影
张杰
古绍彬
Zhao Lina;Xie Yidi;He Zikang;Chai Mengyang;Gao Yuanjingkun;Wu Ying;Zhang Jie;Gu Shaobin(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471000,Henan,China)
出处
《中国沙漠》
北大核心
2025年第3期185-190,共6页
Journal of Desert Research
基金
国家自然科学基金青年科学基金项目(32201383)
河南省重大科技专项项目(231100310200)
河南省重点研发项目(241111314200)。
关键词
生物土壤结皮
碳降解菌株
碳循环
荒漠化
biological soil crusts
carbon-degrading strains
carbon cycle
desertification
