摘要
灵芝的民间药用历史悠久,具有重要的药用价值和经济价值。为充分保护和开发海南热带雨林野生灵芝资源,对采自海南热带雨林国家公园五指山片区的1株野生灵芝菌株X2287进行分离鉴定。利用形态学和TEF1-α-RPB2序列相结合的方法鉴定该菌株为无柄紫灵芝Ganoderma orbiforme。本文对其菌丝的生物学特性及驯化栽培条件进行初步研究。结果表明,菌丝生长最适碳源为蔗糖、最适氮源为氯化铵、最适pH为5.0、最适温度为30℃。在驯化栽培过程中,发菌温度25℃、黑暗条件下菌丝约30 d满袋;原基形成约7–10 d,出原基后约25 d子实体成熟。研究结果可为海南热带雨林特色灵芝资源发掘、新品种选育提供基础数据参考。
Ganoderma,as indigenous traditional medicine with a long application history,has important medical properties and economic values.A wild strain of Ganoderma X2287 collected from Wuzhishan area in National Park of Hainan Tropical Rainforest was isolated and identified as Ganoderma orbiforme by the combination of morphology and TEF1-α-RPB2 sequence.The biological characteristics and domestication conditions of the strain were studied.The results showed that the optimal carbon source for mycelial growth was sucrose;the optimal nitrogen source was ammonium chloride;the optimal pH was 5.0,and the optimal temperature was 30℃.The mycelia fully colonized the media of culture bag after cultivation for about 30 days at 25℃under dark conditions.The primordium began to form in 7–10 days,and the fruiting body matured in 25 days.The results provide basic data for development and utilization of the Ganoderma resources in Hainan Tropical Rainforest and breeding of new varieties.
作者
马思宇
陈爽
王军芳
屈直
刘爽
孙景芳
朱安红
MA Siyu;CHEN Shuang;WANG Junfang;QU Zhi;LIU Shuang;SUN Jingfang;ZHU Anhong(College of Plant Protection,Jilin Agricultural University,Changchun 130118,Jilin,China;Hainan Key Laboratory of Tropical Microbe Resources,Haikou Key Laboratory for Protection and Utilization of Edible and Medicinal Fungi,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang 571339,Hainan,China;School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083,China)
出处
《菌物学报》
北大核心
2025年第7期107-119,共13页
Mycosystema
基金
海南省重点研发计划-海南国家公园研究院资助项目(ZDYF2023RDYL01,KY-24ZK02)
五指山市生态科技特派员项目(WZSKTPXM202211)
中央级公益性科研院所基本科研业务费专项(1630012025308)。
关键词
无柄紫灵芝
分离
鉴定
生物学特性
驯化与栽培
Ganoderma orbiforme
isolation
identification
biological characteristics
domestication and cultivation
