摘要
为促进伽师瓜品种保育及种质创新,本研究以伽师瓜常规种和杂交种的种子为材料,采用无菌播种的方式获得无菌苗,探究不同外植体诱导愈伤组织及不定芽分化的条件。结果表明,2种伽师瓜种子最佳消毒方法是:在超净工作台中去壳后,用70%乙醇浸泡30 s, 3%NaClO溶液消毒3 min。诱导效果最好的外植体是5 d无菌苗的近胚轴端子叶。最佳诱导培养基是基本培养基(MS)+1.0 mg·L^(-1)6-苄基腺嘌呤(6-BA),愈伤组织诱导率达100.0%;伽师瓜常规种不定芽分化率达73.3%,伽师瓜杂交种不定芽分化率达66.7%。
In order to protect the variety and innovate the germplasm of the Jiashi melon(Cucumis melo L.),this study obtained aseptic seedlings through aseptic sowing of common and hybrid Jiashi melon seeds,and explored the conditions for inducing callus tissue and adventitious bud differentiation from different explants.The results showed that the optimal disinfection method for two kinds of Jiashi melon seeds was to soak them in 70%ethanol for 30 seconds and 3%NaClO solution for 3 minutes after shelling them on a clean bench.The best inducing explant was cotyledon of the 5-day aseptic seedling near the hypocotyl.The best induction medium was MS+1.0 mg·L^(-1)6-BA,with a callus tissue induction rate of 100.0%.The adventitious bud differentiation rate of common Jiashi melon was 73.3%,and that of hybrid Jiashi melon was 66.7%.
作者
徐银
方志刚
顾邹壹
吴雨荷
宋刚
XU Yin;FANG Zhigang;GU Zouyi;WU Yuhe;SONG Gang(College of Tea and Food Technology Science,Jiangsu Vacational College of Agriculture and Forestry,Jurong 212400,Jiangsu;Xinjiang Key Laboratory of Biological Resources and Ecology of Pamir Plateau,Kashi 844006,Xinjiang;Modern Agricultural College,Kashi University,Kashi 844006,Xinjiang)
出处
《浙江农业科学》
2025年第7期1642-1646,共5页
Journal of Zhejiang Agricultural Sciences
基金
江苏省高职院校教师专业带头人高端研修项目(2020GRGDYX053)
江苏省大学生创新创业训练项目(202113103026y)
新疆帕米尔高原生物资源与生态重点实验室开放课题项目(XJDX1714-2021-09)。
关键词
伽师瓜
无菌苗
愈伤组织
不定芽
6-BA
Jiashi melon
aseptic seeding
callus tissue
adventitious bud
6-BA
