摘要
目的观察三橘荔核汤调控TLR9/MyD88信号通路治疗Ⅲ型前列腺炎作用机制。方法建立Ⅲ型前列腺炎模型大鼠,将其随机分为空白组,假手术组,模型对照组,对照药物组,三橘荔核汤低、中、高剂量组,给予以相应的药物干预,连续4周。给药结束后处死动物获取前列腺组织,分别进行HE染色观察病理变化,Elisa法检测炎症因子指标,Western blot检测TLR9/MyD88信号通路相关基因蛋白的表达水平,RT-PCR法检测TLR9/MyD88信号通路相关基因表达水平。结果三橘荔核汤可以有效改善Ⅲ型前列腺炎模型大鼠前列腺组织炎性细胞浸润,可以降低模型大鼠前列腺组织相关促炎症因子IL-6、IL-17水平,提高抗细胞因子IL-10水平,药效与剂量呈正相关性。还可以下调TLR9/MyD88信号通路相关基因TLR9、MyD88、IRAK1、IRAK4、TRAF6蛋白与基因的表达,且存在一定的量效关系,使用三橘荔核汤高剂量时效果最佳。结论由实验结果推测三橘荔核汤治疗Ⅲ型前列腺炎可能与其调控TLR9/MyD88信号通路有关。
Objective To observe the mechanism of the regulation of TLR9/MyD88 signaling pathway by Sanjulihe Prescription in the treatment of type Ⅲ prostatitis.Methods Establish the type Ⅲ prostatitis model rats and then randomly divided them into blank group,sham-operated group,model-control group,control drug group,and low,medium,and high dose groups of Sanjulihe Prescription,and were given the appropriate drug interventions for 4 consecutive weeks.At the end of drug administration,the animals were executed to obtain prostate tissues,which were subjected to HE staining to observe pathological changes,Elisa method to detect serum inflammatory factor indexes,Western blot to detect the expression level of genes and proteins related to TLR9/MyD88 signaling pathway,and PCR method to detect the expression level of genes related to TLR9/MyD88 signaling pathway.Results Sanjulihe Prescription can effectively improve the pathological changes of prostate tissue in type Ⅲ prostatitis model rats,and can improve inflammatory cell infiltration.Sanjulihe Prescription could reduce the levels of serum-related inflammatory factors IL-6,IL-17,and increase the level of regulatory cytokine IL-10 in model rats,and the efficacy of the drug showed a positive correlation with the dose.It can also down-regulate the expression of TLR9/MyD88 signaling pathway related genes TLR9,MyD88,IRAK1,IRAK4,TRAF6 proteins and genes,and there is a certain quantitative relationship between the efficacy of the drug and the dose,and the best effect is achieved when using a high dose of Sanjulihe Prescription.Conclusion From the experimental results,it was hypothesized that the treatment of type Ⅲ prostatitis by Sanjulihe Prescription might be related to its modulation of TLR9/MyD88 signaling pathway.
作者
周思怡
朱文雄
刘涛
李博
尉毅
袁轶峰
ZHOU Siyi;ZHU Wenxiong;LIU Tao;LI Bo;WEI Yi;YUAN Yifeng(The First Clinical College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha410208,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha410007,China)
出处
《云南中医药大学学报》
2025年第4期52-58,66,共8页
Journal of Yunnan University of Chinese Medicine
基金
湖南省中医药科研计划重点指导课题(E2022009)
湖南省自然科学基金青年基金项目(2022JJ40335)
湖南省卫生健康委科研计划课题(B202304058417)
湖南省中医药科研计划课题(B2023096)
陈其华全国名老中医药专家传承工作室建设项目
湖南省中医医疗质量控制中心(外科)。
