摘要
为了挖掘龙脑樟中参与龙脑生物合成的磷脂酸磷酸酶基因CcPAP,基于龙脑樟的基因组和转录组数据,利用PCR技术成功克隆出3个CcPAP基因,其开放阅读框长度分别为960、987、957 bp,编码长度分别为320、329、319个氨基酸的蛋白质,且在进化树上与巴豆属植物CsPAP聚为一簇。生物信息学分析显示,CcPAP1为不稳定的疏水性蛋白,而CcPAP3和CcPAP8则为稳定的疏水性蛋白。通过在线软件预测3个CcPAPs蛋白质可能定位于叶绿体上。转录组和qRT-PCR分析结果表明,这3个基因在龙脑樟不同组织中的表达量从茎到根再到叶片逐渐降低,与龙脑二磷酸合成酶CcBPPS具有相似的表达模式,提示3个磷酸酶很有可能参与龙脑樟中龙脑的生物合成。
To explore the phosphatidic acid phosphatase gene CcPAP involved in borneol biosynthesis in Cinnamomum camphora,based on the genomic and transcriptome data of Cinnamomum camphora,of which three CcPAP genes were successfully cloned using PCR technology,with open reading frame lengths of 960987 and 957 bp respectively,encoded proteins with lengths of 320329 and 319 aa respectively,and was clustered together with the CsPAP of the Croton L.plants on the evolutionary tree.Bioinformatics analysis revealed that CcPAP1 was an unstable hydrophobic protein,while CcPAP3 and CcPAP8 were stable hydrophobic proteins.Three CcPAPs proteins were predicted to be located on chloroplasts through online software.The results of transcriptome and qRT-PCR analysis indicated that the expression levels of these three genes in different tissues of Cinnamomum camphora gradually decreased from the stem to the root and then to the leaf,and had a similar expression pattern to the borneol diphosphate synthase CcBPPs ,suggesting that the three phosphatases were very likely to be involved in the biosynthesis of borneol in Cinnamomum camphora.
作者
杨泽锐
杨昕南
郑夏生
YANG Zerui;YANG Xinnan;ZHENG Xiasheng(Key Specialty of Clinical Pharmacy,The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510000,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 511436,China;School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)
出处
《种子》
北大核心
2025年第6期17-22,41,共7页
Seed
基金
国家自然科学基金青年基金项目(82304657)
2024年度基础与应用基础研究专题“青年博士“启航”项目”(2024A04J4895)。
关键词
龙脑樟
磷脂酸磷酸酶
生物合成
龙脑
Cinnamomum camphora
phosphatidic acid phosphatase
biosynthesis
borneol
