摘要
目的解决传统重症急性胰腺炎(SAP)模型的局限性,建立一个操作简便、稳定可靠的动物模型,以促进对SAP发病机制的理解和治疗靶点的发掘。方法采用C57BL/6小鼠,通过雨蛙素预处理结合胰管结扎技术构建SAP模型,设立假手术对照组,评估模型的有效性。运用转录组学、免疫组化、免疫印迹等多种技术,系统分析模型的分子特征及器官损伤情况。结果SAP模型组小鼠显示出典型的SAP病理特征,如血清淀粉酶水平升高、胰腺组织炎症及远端器官损伤,转录组学分析揭示了与SAP关键病理过程相关的基因表达变化,验证了模型的科学价值。结论本研究构建的SAP模型操作简便,具有良好的可重复性,为研究SAP的分子机制提供了一个高效工具,通过揭示潜在的治疗靶标,为该病的治疗策略开发和预后改善奠定了坚实的基础。
Objective To address the limitations of traditional severe acute pancreatitis(SAP)models and establish a simple,stable,and reliable animal model to enhance the understanding of SAP pathogenesis and facilitate the discovery of therapeutic targets.Methods C57BL/6 mice were pretreated with caerulein followed by pancreatic duct ligation to establish the SAP model.A sham surgery control group was also set up to assess the model′s effectiveness.The molecular characteristics and organ damage of the model were evaluated by transcriptomics,immunohistochemistry,and Western blot.Results Mice in the model group exhibited typical SAP pathological features,including elevated serum amylase levels,pancreatic inflammation,and damage to distant organs.Transcriptomic analysis revealed gene expression changes associated with key pathological processes of SAP,validating the scientific value of the model.Conclusions The proposed SAP model is easy to operate,highly reproducible,and provides an efficient tool for investigating the molecular mechanisms of SAP.By revealing potential therapeutic targets,it lays a solid foundation for the development of treatment strategies and the improvement of prognosis for this disease.
作者
周全
李洪祯
王烜
施照
沈珊珊
邹晓平
ZHOU Quan;LI Hongzhen;WANG Xuan;SHI Zhao;SHEN Shanshan;ZOU Xiaoping(Department of Gastroenterology,the Affiliated Drum Tower Hospital,Medical School of Nanjing University,Nanjing,Jiangsu 210000,China)
出处
《徐州医科大学学报》
2025年第7期477-483,共7页
Journal of Xuzhou Medical University
基金
国家自然科学基金面上项目(82203657)。
关键词
重症急性胰腺炎
小鼠模型
雨蛙素
胰管结扎
转录组学分析
severe acute pancreatitis
mouse model
caerulein
pancreatic duct ligation
transcriptomic analysis
