摘要
活细胞中低水平miRNA检测的扩增策略对于肿瘤生物标志物的精准检测具有重要意义。因此在本工作中设计了一种在活细胞内利用h-BNNS和四向催化发夹组装(FCHA)技术进行miRNA-21成像的扩增策略。具有低细胞毒性的h-BNNS作为优异的载体和荧光猝灭剂,将修饰有FAM的发夹DNA递送至细胞内。在细胞内miRNA-21的存在下,触发FCHA反应形成十字型DNA结构,以减弱DNA和h-BNNS之间的作用力,同时在一个DNA纳米产物上实现多个荧光信号的标记提高了检测的准确度。这种方法为活细胞内miRNA的超灵敏检测提供了强有力的工具,检测限低至0.5 pmol·L^(-1)。
The amplification strategy of low level miRNA detection in living cells is of great sig-nificance for the accurate detection of tumor biomarkers.This work was designed an amplifica-tion strategy for miRNA-21 imaging using h-BNNS and four-way catalytic hairpin assembly(FCHA)in living cells.As an excellent carrier and fluorescence quencher,h-BNNS with low cytotoxicity delivers hairpin DNA modified with FAM into cells.In the presence of miRNA-21,the FCHA reaction is triggered to form a cross DNA structure for weakening the force between DNA and h-BNNS.At the same time,the labeling of multiple FAM signal on a DNA nano-product improves the detection accuracy.This method provides a powerful tool for the ultra-sensitive detection of miRNA in living cells,and the detection limit is as low as 0.5 pmol·L^(-1).
作者
李甜甜
何鹏
李东祥
LI Tiantian;HE Peng;LI Dongxiang(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China)
出处
《青岛科技大学学报(自然科学版)》
2025年第4期12-20,共9页
Journal of Qingdao University of Science and Technology(Natural Science Edition)
基金
国家自然科学基金项目(21975139)。
