摘要
目的评价7种非灭活型病毒保存液对病毒的核酸和活性的的保存效果。方法对7种市售非灭活型病毒保存液,分别添加等量甲型H1N1流感病毒,于-20℃、4℃、25℃和37℃条件下保存1 h、6h、1天、3天和5天。通过实时荧光定量PCR检测各模拟样本在不同保存条件下的病毒核酸载量。通过病毒分离培养及红细胞凝集试验测定血凝(hemagglutination,HA)滴度,比较不同保存液在不同保存条件下病毒生长活性的差异。结果除E外,其余保存液在4种保存温度下均能较好地保护病毒核酸。在病毒活性方面,A、B、C和D均能有效保持病毒活性。A和B的效果最好,E和F的效果较差,G的效果最差。结论大多数的非灭活型病毒保存液对病毒核酸的保护效果较好,但在保持病毒活性方面存在较大差异。为保证病毒的保存效果,建议医疗机构在使用前进行保存效果评价。
Objective To evaluate the preservation efficacy of 7 non-inactivating virus preservation solutions.Methods Equal amounts of H1N1 virus were added to 7 commercially available non-inactivating virus preservation solutions,and the samples were stored at-20℃,4℃,25℃and 37℃for 1 hour,6 hours,1 day,3 days,and 5 days.The viral nucleic acid in each simulated sample under different storage conditions was measured using real-time quantitative PCR.The hemagglutination(HA)titer was determined through viral isolation culture and hemagglutination assay,comparing the differences in viral growth activity across different storage solutions and conditions.Results Except for solution E,the other solutions effectively protected viral nucleic acid at the 4 storage temperatures.In terms of viral activity,solutions A,B,C,and D effectively maintained viral viability.A and B showing the best performance,E and F showed poorer performance,and G performed the worst.Conclusions Most non-inactivating virus preservation solutions effectively protect viral nucleic acid,but there are significant differences in their ability to maintain viral viability.To ensure optimal virus preservation,it is recommended that medical institutions evaluate the effectiveness of preservation solutions before use.
作者
高群
吴丹
赵佳琛
张莉
王宇
刘医萌
卢桂兰
彭晓旻
段玮
张代涛
王全意
石伟先
Gao Qun;Wu Dan;Zhao Jiachen;Zhang Li;Wang Yu;Liu Yimeng;Lu Guilan;Peng Xiaomin;Duan Wei;Zhang Daitao;Wang Quanyi;Shi Weixian(Institute of Infectious Disease and Endemic Disease Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China)
出处
《中华实验和临床病毒学杂志》
2025年第3期383-387,共5页
Chinese Journal of Experimental and Clinical Virology
基金
北京市科技计划(241100009024047)。
关键词
非灭活型
病毒保存液
H1N1
HA滴度
效果评价
Non-inactivating
Viral preservation solution
H1N1
HA titer
Evaluation of the preservation effects
