摘要
为实现枇杷叶精油(LLEO)的可控释放,采用复合凝聚法制备枇杷叶精油微胶囊(M-LLEO),通过测定包埋率等理化指标,并结合一系列表征技术,评价微胶囊化的可行性;通过贮藏稳定性、释放动力学、自由基清除及抑菌试验综合评价其生物活性,并将其应用于蓝莓的保鲜试验。结果表明,M-LLEO的包埋率和得率分别为86.39%,62.02%;M-LLEO呈现大小不一的球状颗粒形态,壁材无破裂痕迹;LLEO以物理吸附的方式被成功包埋;微胶囊具有较高的热稳定性;M-LLEO适合在低温、避光、无氧及低湿条件下贮藏;在较高温度下,精油的释放曲线与一级动力学模型拟合效果最好(R^(2)>0.99);在第10 d时,相较于LLEO,M-LLEO对DPPH和ABTS^(+)·的清除率分别提高40%,35.93%;相较于M-LLEO对培养24 h的大肠杆菌及巨大芽孢杆菌平板产生的抑菌圈直径,培养72 h产生的抑菌圈直径分别增大5.49,4.29 mm,说明微胶囊的缓释作用使得精油的抗氧化性和抑菌效果较为持久。低温结合微胶囊处理可降低蓝莓的失重率,延缓硬度、可滴定酸含量的下降;贮藏10 d后,蓝莓的可溶性固形物含量较室温对照组高41.9%。研究为LLEO的拓展应用提供理论和实践依据。
To achieve controlled release of loquat leaf essential oil(LLEO),microencapsulated LLEO(M-LLEO)was prepared via complex coacervation.The feasibility of microencapsulation was evaluated by measuring encapsulation efficiency and physicochemical indices combined with characterization techniques.Bioactivity was comprehensively assessed through storage stability,release kinetics,free radical scavenging,and antibacterial tests,followed by preservation trials on blueberries.Results showed encapsulation efficiency and yield of M-LLEO reached 86.39%and 62.02%,M-LLEO exhibited spherical particle morphologies with diverse size distributions.There were no signs of cracking on the wall material.LLEO was successfully embedded by physical adsorption.Microcapsules demonstrated high thermal stability.M-LLEO was suitable for storage at low temperature,darkness,anaerobic,and low-humidity conditions.At elevated temperatures,release kinetics best fitted the first-order model(R^(2)>0.99).On day 10,M-LLEO increased DPPH and ABTS^(+)·scavenging rates by 40%and 35.93%versus free LLEO.Inhibition zone diameters against E.coli and Bacillus megaterium increased by 5.49 mm and 4.29 mm at 72 h versus 24 h.This indicated that the sustained-release effect of microcapsules maked the antioxidant and antibacterial effects of essential oils more lasting.Low-temperature combined with M-LLEO treatment reduced blueberry weight loss rate while delaying declines in hardness and titratable acid.After 10 days storage,soluble solids content of treated blueberries exceeded room-temperature controls by 41.9%.This study provides theoretical and practical foundations for LLEO applications.
作者
方超逸
沈建东
邓仕彬
周凤超
马逸芬
FANG Chaoyi;SHEN Jiandong;DENG Shibin;ZHOU Fengchao;MA Yifen(College of Environmental and Biological Engineering,Putian University,Putian 351100,China;Key Laboratory of Ecotoxicological Effects and Control of New Pollutants in Fujian Province,Putian 351100,China;Key Laboratory of Ecological Environment and Information Mapping of Fujian Provincial Universities,Putian 351100,China)
出处
《包装与食品机械》
北大核心
2025年第3期22-34,共13页
Packaging and Food Machinery
基金
福建省自然科学基金项目(2023J011005,2022J011161)
福建省教育厅中青年教师教育科研项目(JAT200506)。
关键词
枇杷叶精油
微胶囊
表征
生物活性
蓝莓保鲜
loquat leaf essential oil
microencapsulation
characterization
bioactivity
blueberry preservation
