摘要
为明确烟草TIR1/AFBs家族基因对生长素类除草剂的抗性,阐明二氯喹啉酸药害的机理,对烟草生长素受体基因家族进行鉴定,并对基因表达模式进行分析。结果表明,普通烟草存在10个NtTIR1/AFBs基因。亚细胞定位分析显示,有5个NtTIR1/AFBs定位在细胞核,3个定位在细胞质,2个定位在叶绿体。进化分析表明,NtTIR1/AFBs进化分为4类,拟南芥的AtTIR1/AFBs分为3类。蛋白结构域分析显示,NtTIR1/AFBs主要含有F-box和LRR保守结构域。在结构域的数量上,NtTIR1/AFBs和AtTIR1/AFBs存在差异。MicroRNA靶点分析发现,4个NtTIR1/AFBs含有潜在的miRNA靶点,其中20083和20090基因均含有8个miRNA靶点。组织表达分析表明,20081、20082、20088和20091 4个基因在烟草各组织中的表达量相对较高。二氯喹啉酸处理后基因表达分析显示,20085和20086基因在烟草根系中上调表达幅度较大,20082基因在烟草叶片中上调幅度最大,而20081和20090基因在烟草叶片中下调幅度较大。
To elucidate the resistance of NtTIR1/AFBs family genes to auxin herbicides and the mechanismof quinclorac phytotoxicity in tobacco, the tobacco auxin receptor gene family was identified and theirgene expression patterns were analyzed. The results showed that there were ten NtTIR1/AFBs genes inNicotiana tabacum. Subcellular localization analysis showed that five NtTIR1/AFBs localize in the nucleus,three in the cytoplasm, and two in the chloroplasts. Phylogenetic analysis revealed that NtTIR1/AFBs were evolved into four classes, while AtTIR1/AFBs in Arabidopsis thaliana into three classes. Protein domainanalysis revealed the presence of conserved F-box and LRR domains predominantly in NtTIR1/AFBs.Furthermore, there were differences in the number of domains between NtTIR1/AFBs and AtTIR1/AFBs.MicroRNA target analysis identified putative miRNA binding sites within four NtTIR1/AFBs genes, with20083 and 20090 harboring eight miRNA target sites each. Tissue expression analysis revealed that theexpressions of 20081, 20082, 20088 and 20091 were relatively high in all tissues. Notably, geneexpression analysis after quinclorac treatment showed that the expressions of 20085 and 20086 werehighly up-regulated in tobacco roots. 20082 had the highest up-regulated expression fold in tobacco leaves,while 20081 and 20090 had the highest down-regulated expression fold in tobacco leaves.
作者
高军平
杨小年
谢小东
朱丽颖
李冰洁
李锋
黄平俊
何鑫玺
李泽锋
王帅斌
陈凯
罗朝鹏
吕婧
孙玉合
杨军
GAO Junping;YANG Xiaonian;XIE Xiaodong;ZHU Liying;LI Bingjie;LI Feng;HUANG Pingjun;HE Xinxi;LI Zefeng;WANG Shuaibin;CHEN Kai;LUO Zhaopeng;LÜJing;SUN Yuhe;YANG Jun(Technology Center,China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410007,China;Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Tobacco Research Institute of CAAS,Qingdao 266101,Shandong,China)
出处
《烟草科技》
北大核心
2025年第7期35-43,共9页
Tobacco Science & Technology
基金
湖南中烟工业有限责任公司科技项目“烟草二氯喹啉酸类除草剂抗性基因筛选与利用”(KY2020YC0003)、“基于重离子辐射的K326突变体库创制与种质筛选”(KY2024YC0019)
中国烟草总公司烟草基因组计划与生物育种重大科技项目“烟草抗二氯喹啉酸稻田除草剂药害的突变基因克隆与功能研究”[110202101036(JY-13)]
湖南中烟-郑州烟草研究院烟草基因功能研究与利用联合实验室项目。
关键词
烟草
生长素受体
二氯喹啉酸药害
基因家族
Nicotiana tabacum
Auxin receptor
Quinclorac phytotoxicity
Gene family
