摘要
为探究南瓜果胶含量的遗传调控机制,挖掘关键基因,为南瓜口感品质的遗传改良提供理论依据,该研究采用硫酸-咔唑比色法测定208份南瓜种质资源果肉的果胶含量,利用果胶含量数据与重测序获得的单核苷酸多态性(single nucleotide polymorphism, SNP)进行全基因组关联分析(genome-wide association study, GWAS),对显著关联区间进行候选基因筛选与功能注释。结果显示,南瓜果胶含量呈正态分布,在南瓜2、4、12、20号染色体上共获得了21个与南瓜果胶含量显著关联的SNP位点,其中有13个位于2号染色体8330664~8436445区间。筛选获得40个候选基因,其中3个基因(基因号为CmoCh02G014160、CmoCh02G014170、CmoCh02G014260)涉及糖代谢通路。这3个候选基因在南瓜种质资源中存在着大量的SNP和InDel变异,并且在南瓜果实中均有表达,可能参与南瓜果实中果胶的合成。该研究结果可以为优化南瓜果胶含量,改良南瓜品质提供新的标记与候选基因。
To investigate the genetic regulatory mechanisms of pectin content in pumpkin and identify key genes,and provide a theoretical basis for the genetic improvement of pumpkin fruit texture quality,pectin content of 208 pumpkin germplasm resources was determined using sulfuric acid-carbazole method,a genome-wide association study(GWAS)was conducted by integrating the pectin content data with single nucleotide polymorphisms(SNP)obtained from whole-genome resequencing,candidate gene screening and functional annotation were performed in the significantly associated genomic regions.The results revealed that pumpkin pectin content followed a normal distribution,21 SNPs significantly associated with pumpkin pectin content were identified on chromosomes 2,4,12,and 20,with 13 significant SNPs located in 8330664-8436445 on chromosome 2.Totally,40 candidate genes were screened,with three genes(gene ID were CmoCh02G014160,CmoCh02G014170 and CmoCh02G014260)annotated to be involved in sugar metabolism pathways.These three candidate genes had a large number of SNP and InDel variations in the pumpkin germplasm resources,and were expressed in pumpkin fruits,they may be involved in pectin biosynthesis in pumpkin fruits.This study can provides new molecular markers and candidate genes for optimizing pectin content and improving pumpkin quality through genetic breeding.
作者
陶鹏
丁小雅
岳智臣
赵彦婷
雷娟利
胡齐赞
臧运祥
李必元
TAO Peng;DING Xiaoya;YUE Zhichen;ZHAO Yanting;LEI Juanli;HU Qizan;ZANG Yunxiang;LI Biyuan(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,China)
出处
《浙江农业学报》
北大核心
2025年第6期1244-1251,共8页
Acta Agriculturae Zhejiangensis
基金
浙江省农作物种质资源精准鉴定评价任务(南瓜)(2022R23T60D01)。
关键词
南瓜
果胶
全基因组关联分析
基因
糖代谢
pumpkin
pectin
genome-wide association study
gene
sugar metabolism
