摘要
目的观察SD大鼠体质量和体长对颈总动脉球囊拉伤致血管狭窄模型的影响,为复制理想的血管狭窄模型提供参考。方法将雄性SD大鼠按体质量和体长分为3组。选用CONQUEROR TM SC PTCA球囊导管,固定球囊充盈量为0.2 mL,拉伤大鼠左侧颈总动脉,以健侧为对照。术后即刻每组选1只大鼠通过Evans Blue染液验证,其余大鼠于14 d后取损伤侧和健侧颈总动脉进行HE染色判断其狭窄情况,并基于此,选择理想体质量和体长范围内的6只大鼠进行重复验证。结果体质量在280~380 g(相应体长为21.0~26.5 cm)范围内的大鼠,经球囊导管拉伤术后,均致颈总动脉内膜脱落和不同程度的狭窄。其中体质量280~300 g(相应体长为21.0~22.5 cm)大鼠颈总动脉严重狭窄甚至伴有血栓闭塞;体质量320~340 g(相应体长为23.0~24.5 cm)大鼠见颈总动脉内外弹力板断裂,血管形态异常;而体质量360~380 g(相应体长为25.0~26.5 cm)大鼠颈总动脉未见弹力板断裂和血栓,且体质量为360 g的小鼠血管狭窄程度适中且均一,重复验证试验结果一致。结论选用体质量为360 g(相应体长为25.0~26.5 cm)的大鼠可复制出较为理想的血管狭窄模型。
Objective To investigate the effects of body weight and length of SD rats on the carotid artery balloon injury-induced vascular stenosis model in order to provide a reference for replicating an ideal vascular stenosis model.Methods Male rats were divided into three groups based on body weight and length.The CONQUEROR TM SC PTCA balloon catheter was employed,with a fixed balloon inflation volume of 0.2 mL to induce injury in the left common carotid artery,while the right side served as a control.As soon as surgery operation,one rat from each group was selected for Evans Blue dye verification.Fourteen days later,the injured and contra lateral common carotid arteries from remaining rats were harvested for HE staining to check the extent of stenosis.Based on these findings,six rats within the optimal range of body weight and length were selected for further validation.Results Rats with body weights ranging from 280 to 380 g(corresponding body lengths of 21.0-26.5 cm)underwent balloon catheter injury,resulting in endothelial detachment and varying degrees of stenosis in the common carotid artery.In rats weighing 280-300 g(body lengths of 21.0-22.5 cm)had severe stenosis or occlusion of the common carotid artery with thrombosis.In rats weighing 320-340 g(body lengths of 23.0-24.5 cm),the internal and external elastic plates of the common carotid artery were ruptured and the vascular morphology was abnormal.Conversely,rats weighing 360-380 g(body lengths of 25.0-26.5 cm)did not show any ruptured elastic laminae or thrombus formation in the common carotid artery,and the extent of vascular stenos in rats with a body weight of 360 g was moderate and uniform.The results of the repeated validation experiments were consistent.Conclusions Rats with a body weight range of 360 g(corresponding body length of 25.0-26.5 cm)are suitable for development of an ideal vascular stenosis model.
作者
陈天旺
罗加伟
袁乙丹
杨依敏
黄波
徐尚福
李利生
CHEN Tianwang;LUO Jiawei;YUAN Yidan;YANG Yimin;HUANG Bo;XU Shangfu;LI Lisheng(Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education;Department of Pharmacology,Key Laboratory of Basic Pharmacology of Guizhou Province,Zunyi Medical University,Zunyi 563000;Key Laboratory of Cell Engineering of Guizhou Province,the Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China)
出处
《基础医学与临床》
2025年第8期1034-1040,共7页
Basic and Clinical Medicine
基金
贵州省科技计划项目(黔科合基础-ZK[2023]一般500)。
