摘要
The integration of microorganisms and photosensitizers presents a promising approach to chemical production utilizing solar energy.However,the current system construction process remains complex.Herein,we introduce a straightforward and efficient solar-to-chemical conversion system that combines the dissolved photosensitizer Eosin Y with the non-photosynthetic bacterium Moorella thermoacetica.Under light radiation,acetate production increased to 5.1μM h^(-1)μM^(-1)catalyst,exceeding the previously reported maximum by 5.9-fold,with a quantum efficiency of 17.6%.The soluble photosensitizer EY can penetrate the cell and directly engage in intracellular energy metabolism,significantly enhancing intracellular ATP and NADPH/NADP^(+)levels.Within this biohybrid system,sacrificial agent triethanolamine played a dual role:(1)providing continuous photoelectron generation by Eosin Y,enhancing intracellular reducing power,and facilitating carbon fixation via the Wood-Ljungdahl pathway;and(2)its oxidation product,formaldehyde,served as a critical intermediate and a direct precursor for methylenetetrahydrofolate in the Wood-Ljungdahl pathway,consequently simplifying reaction steps and markedly boosting acetate yield.This study provides a simple microorganism-photosensitizer biohybrid system to produce acetate and light on the multifaceted roles of sacrificial agents,paving the development of efficient solar energy conversion with nonphotosynthetic bacteria.
基金
supported by the National Natural Science Foundation of China(22236007,22025603,21802133)
the Natural Science Foundation of Xiamen,China(3502Z20227241)。
