摘要
目的探索环状RNA(circRNA)hsa_circ_0002938在胃癌中的表达及其生物学功能。方法收集入院手术切除的45例胃癌患者的癌组织和相应癌旁组织,同时常规培养人胃癌细胞SGC-7901、BGC-823、HGC-27和MGC-803以及永生化胃黏膜上皮细胞GES-1。采用实时荧光定量逆转录聚合酶链反应(RT-qPCR)检测hsa_circ_0002938在胃癌组织和细胞中的表达水平,利用卡方检验分析其与患者临床病理特征之间的关系,Kaplan-Meier生存分析评估其表达水平与患者预后的相关性。细胞计数试剂盒8(CCK-8)、划痕愈合实验、Transwell实验检测hsa_circ_0002938对胃癌细胞增殖、迁移和侵袭能力的影响。进一步利用生物信息学分析预测hsa_circ_0002938可能结合的微小RNA(miRNA)及其下游的靶基因,构建竞争性内源RNA(ceRNA)调控网络,并对靶基因进行功能富集。结果hsa_circ_0002938在胃癌组织和胃癌细胞中的表达水平高于癌旁组织和GES-1细胞(P<0.05),hsa_circ_0002938高表达组患者术后2年无进展生存期缩短(P<0.01)。敲低hsa_circ_0002938后,胃癌细胞的增殖、迁移和侵袭能力均下降(P<0.05)。生物信息学预测分析显示hsa_circ_0002938可结合hsa-miR-342-3p和hsa-miR-503-5p,功能富集结果显示miRNA下游靶基因富集于丝裂原活化蛋白激酶(MAPK)、Hippo及Wnt等多个癌症相关信号通路。结论在胃癌组织和细胞中呈高表达的hsa_circ_0002938与患者的不良预后密切相关,敲低hsa_circ_0002938可抑制胃癌细胞的增殖、迁移和侵袭能力。研究揭示了hsa_circ_0002938在胃癌进展中的潜在作用,为胃癌的防治提供了新思路。
Objective To investigate the expression and biological function of circular RNA(circRNA)hsa_circ_0002938 in gastric cancer.Methods 45 pairs of tumor tissues and para-cancerous tissues from gastric cancer patients who underwent tumor reduction surgery at the hospital were collected.Human gastric cancer cells SGC-7901,BGC-823,HGC-27,MGC-803 and immortalized gastric mucosal epithelial cells GES-1 were routinely cultured.RT-qPCR was utilized to detect the expression of hsa_circ_0002938 in gastric cancer tissues and cells.Chi-square test was used to analyze its association with the clinicopathological characteristics of patients,and the Kaplan-Meier method was employed to assess the relationship between hsa_circ_0002938 expression levels and patient prognosis.The effects of hsa_circ_0002938 on the proliferation,migration and invasion abilities of gastric cancer cells were detected by CCK-8,scratch wound healing and Transwell assays.Bioinformatics was used to predict the miRNA and its downstream target genes that hsa_circ_0002938 might bind to.The competitive endogenous RNA regulatory network was constructed and the functions of the target genes were enriched.Results The expression of hsa_circ_0002938 in gastric cancer tissues and cells was much higher than that in para-cancerous tissues and GES-1 cells(P<0.05).By contrast,patients in the high hsa_circ_0002938 expression group had significantly shorter 2-year progression-free survival than that in the low expression group after surgery(P<0.01).Moreover,knocking down the expression of hsa_circ_0002938 reduced the proliferative,migratory,and invasive abilities of gastric cancer cells(P<0.05).Bioinformatics predictive analysis showed that hsa_circ_0002938 could bind to hsa-miR-342-3p and hsa-miR-503-5p.The downstream target genes of miRNA were involved in several cancer-related pathways,like mitogen-activated protein kinase(MAPK),hippo and Wnt signaling pathway.Conclusion Hsa_circ_0002938,which is highly expressed in gastric cancer tissues and cells,is closely associated with poor patient prognosis.Knockdown of hsa_circ_0002938 inhibits the proliferation,migration,and invasion abilities of gastric cancer cells.The study reveals the potential role of hsa_circ_0002938 in the progression of gastric cancer,offering new insights into its prevention and treatment.
作者
李晓鹏
刘思涵
赵一杰
李书梅
殷小磊
Li Xiaopeng;Liu Sihan;Zhao Yijie;Li Shumei;Yin Xiaolei(Dept of Gastroenterology,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050035;Dept of Medical Records,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050035;Dept of Radiation Therapy,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050035;Dept of Gastrointestinal Surgery,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050035)
出处
《安徽医科大学学报》
北大核心
2025年第6期1043-1051,共9页
Acta Universitatis Medicinalis Anhui
基金
河北省医学科学研究重点课题(编号:20230820)。
