摘要
目的探究使用不同剂量万古霉素(VCM)建造SD大鼠急性肾损伤(AKI)模型的方法及其评价。方法6周龄雄性SD大鼠随机分为4组,正常对照(NC)组、VCM(200、400、600)mg/kg组;腹腔注射给药1次/d,连续7 d;检测大鼠血清尿素氮(BUN)、肌酐(Cre)水平;取肾脏称重,计算肾体比;肾组织进行HE及PAS染色,光镜下观察肾脏病理损伤程度。结果随着VCM剂量的增加,各剂量组大鼠血清BUN和Cre水平呈升高趋势(F=3.85,P<0.05;F=9.02,P<0.01),且VCM 600 mg/kg组与VCM(200、400)mg/kg组相比Cre水平最高,差异具有统计学意义(P<0.01,P<0.05)。随着VCM剂量的增加,各剂量组大鼠体质量呈下降趋势(F=54.81,P<0.01),肾总重和肾体比呈上升趋势(F=11.13,F=14.43,均P<0.01),且VCM 600 mg/kg组与VCM(200、400)mg/kg组相比,大鼠体质量、肾总重、肾体比差异均有统计学意义(P<0.01,P<0.05)。HE染色和PAS染色显示,各剂量组肾小管出现不同程度的损伤,随着VCM剂量的增加,肾损伤评分升高(F=105.80,F=28.89,均P<0.01),且VCM 600 mg/kg组肾小管上皮细胞脱落、肿胀、管型形成等病理损伤最严重。结论VCM 600 mg/kg以1次/d、连续7 d腹腔注射可稳定建立大鼠AKI模型。
Objective To investigate the method and evaluation of using different doses of vancomycin(VCM)to construct an acute kidney injury(AKI)model in SD rats.Methods The 6-week-old male SD rats were randomly divided into 4 groups:normal control(NC)group,VCM(200,400,600)mg/kg group.The drug was given by intraperitoneal injection once a day for 7 days.The levels of blood urea nitrogen(BUN)and creatinine(Cre)were detected.The kidney was weighed and the kidney body ratio was calculated.Renal tissue was stained with HE and PAS,and the pathological injury of kidney was observed under light microscope.Results With the increase of VCM dose,serum BUN and Cre levels in each dose group showed an upward trend(F=3.85,P<0.05;F=9.02,P<0.01).Compared with the VCM(200,400)mg/kg groups,the Cre level in the VCM 600 mg/kg group was the highest,with statistically significant differences(P<0.01,P<0.05).As the VCM dose increased,body weight of rats in each dose group decreased(F=54.81,P<0.01),while total kidney weight and kidney body ratio increased(F=11.13,F=14.43,both P<0.01).Significant statistically differences(P<0.01,P<0.05)were observed in body weight,total kidney weight and kidney body ratio between the VCM 600 mg/kg group and the VCM(200,400)mg/kg groups.HE staining and PAS staining showed that renal tubules in each dose group were damaged to varying degrees.With the increase of VCM dose,the pathological score of kidney injury increased(F=105.80,F=28.89,both P<0.01),and the pathological damage of renal tubular epithelial cells such as shedding,swelling and cast formation was the most serious in VCM 600 mg/kg group.Conclusion The AKI model in rats can be stably established by intraperitoneal injection of VCM at 600 mg/kg once a day for 7 days.
作者
马菲
邓琴香
施瑞
王春
徐丙发
Ma Fei;Deng Qinxiang;Shi Rui;Wang Chun;Xu Bingfa(School of Pharmaceutical Sciences,Anhui Medical University,Hefei 230032;Dept of Pharmacy,The Third Affiliated Hospital of Anhui Medical University,Hefei 230032)
出处
《安徽医科大学学报》
北大核心
2025年第6期1030-1035,共6页
Acta Universitatis Medicinalis Anhui
基金
安徽省重点研究与开发计划(编号:2023s07020003)
安徽医科大学第三附属医院基础与临床合作研究提升计划培育专项(编号:2022sfy016)。
