摘要
目的:检测2款人参再造膏的保健功能。方法:通过小鼠力竭游泳实验并测定疲劳相关的生理指标,以及斑马鱼免疫功能相关指标,评估再造膏A的抗疲劳和增强免疫功能;通过测定小鼠失血前后血常规及斑马鱼活性氧数量变化,检测再造膏B的抗失血和抗氧化作用。结果:再造膏A可以显著增长小鼠力竭游泳时间(P<0.05),显著提高小鼠血糖、肝糖原和肌糖原水平,降低尿素氮、肌酸激酶和乳酸水平(P<0.05);斑马鱼巨噬细胞吞噬功能及中性粒细胞、吞噬细胞和T细胞数量也显著提高(P<0.01)。再造膏B可以显著改善小鼠失血状态,逆转脏器代偿性肥大,增加斑马鱼体内活性氧数量(P<0.01)。结论:再造膏A可以改善疲劳,提高免疫力;再造膏B改善血虚症状,发挥抗氧化功能。
Objective:To evaluate the health functions of two types of Renshenzaizao Paste.Methods:The anti-fatigue and immune-enhancing effects of Paste A were assessed using a mouse exhaustive swimming test and measurement of fatigue-related physiological indices,as well as immune-related indices in zebrafish.The anti-hemorrhagic and antioxidant effects of Paste B were evaluated by analyzing changes in hematological parameters in mice before and after blood loss and by measuring reactive oxygen species(ROS)levels in zebrafish.Results:Paste A significantly prolonged exhaustive swimming time in mice(P<0.05),increased blood glucose,liver glycogen,and muscle glycogen levels,and decreased blood urea nitrogen,creatine kinase,and lactic acid levels(P<0.05).It also significantly enhanced zebrafish macrophage phagocytic activity and increased the numbers of neutrophils,phagocytes,and T cells(P<0.01).Paste B significantly improved hemorrhagic conditions in mice,reversed compensatory organ hypertrophy,and increased ROS levels in zebrafish(P<0.01).Conclusion:Paste A can alleviate fatigue and enhance immune function,while Paste B improves symptoms of blood deficiency and exerts antioxidant effects.
作者
吕明玥
郑彦楠
高建平
王永伟
贾晓薇
丁旭彦
李燕
袁满
LYU Mingyue;ZHENG Yannan;GAO Jianping;WANG Yongwei;JIA Xiaowei;DING Xuyan;LI Yan;YUAN Man(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Tonghan Chuntang Pharmaceutical Co.,Ltd.,Shanghai 200012,China)
出处
《世界中医药》
北大核心
2025年第10期1679-1685,共7页
World Chinese Medicine
基金
国家自然科学基金项目(82204723)——益母草总生物碱抑制HIF-1α介导的MCs活化抗过敏性哮喘机制研究。
关键词
人参再造膏
保健功能
不同人群
亚健康
抗疲劳
免疫
抗氧化
血虚
Renshenzaizao Paste
Health function
Different populations
Suboptimal health
Anti-fatigue
Immune
Antioxidation
Blood deficiency
