摘要
采用层层组装法合成了分散性良好的SiO2@SiO2表面多孔微球(SPP),并将其用作高效液相色谱填料。利用扫描电子显微镜、N_(2)吸附-脱附、热重分析仪对该填料进行表征,10次涂覆后的硅球壳层厚度为500 nm,比表面积为128 m^(2)/g,孔为径10 nm。将其与C18键合后对其分析性能进行测试,在甲醇/水为流动相、流速为0.3 mL/min条件下,各分析物的保留因子随甲醇含量的增加而减小,符合反相保留特征,对多环芳烃的分析具有良好的稳定性。对烷基苯的分析测试中,与粒径相似的全多孔硅球相比,该表面多孔微球填料能有效降低柱压,缩短分析时间。
SiO_(2)@SiO_(2)superficially porous spheres(SPP)with good dispersion were synthesized by using the layer-bylayer method and used as fller in high-performance liquid chromatography.The SiO,@SiO,SPP was characterized by scanning electron microscopy,N2 adsorption-desorption,and thermogravimetric analysis.The silica spheres exhibited a shell thickness of 500 nm,a specific surface area of 128 m'/g,and a pore diameter of 10 nm after ten times coating.The analytical performance of the SPPs was tested after being bonded with C18.The retention factor of each analyte exhibited a decrease with the increasing methanol content at a flow rate of 0.3 mL/min in a methanol/water system,which was consistent with reversed-phase retention characteristics.In the separation test of alkylbenzenes,compared with fully porous silicon spheres,the prepared microspheres can effectively reduce the column pressure and shorten the analysis time.
作者
肖迪
王硕
顾一鸣
王树东
XIAO Di;WANG Shuo;GU Yiming;WANG Shudong(National Laboratory for Clean Energy,Dalian Institute of Chemical Physics Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《分析试验室》
北大核心
2025年第6期939-944,共6页
Chinese Journal of Analysis Laboratory
关键词
层层组装法
表面多孔微球
高效液相色谱
layer-by-layer assembly
superficially porous spheres
high-performance liquid chromatography
