摘要
为了解吉林省牛嗜血支原体的流行情况,试验于2023年7—9月份采集吉林省7个地区(四平市、辽源市、通化市、白山市、舒兰市、松原市、珲春市)牛血液样本共178份,提取血液DNA后,对牛嗜血支原体16S rDNA基因进行PCR扩增与测序,并随机选取部分牛嗜血支原体阳性样本的测序结果进行遗传进化分析;采用卡方检验分别对不同地区、不同年龄、不同饲养方式的牛血液样本中温氏支原体和牛嗜血支原体待定种阳性率进行比较分析。结果表明:从178份血液样本中共检测出嗜血支原体阳性样本79份。其中温氏支原体阳性样本58份,阳性率为32.6%(58/178);牛嗜血支原体待定种阳性样本21份,阳性率为11.8%(21/178);无混合感染。温氏支原体和牛嗜血支原体待定种分别位于系统进化树的两大分支上。温氏支原体Mw-1与河南株Fengdu(FJ375309.1)位于同一小分支,核苷酸相似性为100%,亲缘关系最近;温氏支原体Mw-2与墨西哥株INIFAP01(KX171205.1)位于同一小分支,核苷酸相似性为99.5%,亲缘关系最近;牛嗜血支原体待定种Cm-1与马来西亚株Clone192 4712(KT985638.1)位于同一小分支,核苷酸相似性为100%,亲缘关系最近。温氏支原体阳性率为四平市最高(73.3%,14/19),舒兰市最低(5.3%,1/19);牛嗜血支原体待定种阳性率为珲春市最高(25.0%,10/40),四平市和辽源市阳性率最低(0,0/19;0,0/20)。不同地区之间温氏支原体阳性率均差异显著(P<0.05);除四平市和辽源市牛嗜血支原体待定种阳性率差异不显著(P>0.05)外,其他地区牛嗜血支原体待定种阳性率均差异显著(P<0.05)。<1岁、≥1~<3岁、≥3岁牛温氏支原体阳性率分别0(0/6)、25.0%(22/88)、42.9%(36/84),牛嗜血支原体待定种牛阳性率分别为0(0/6)、10.2%(9/88)、14.2%(12/84),均随年龄的增加而升高。不同年龄牛之间温氏支原体阳性率和牛嗜血支原体待定种阳性率均差异显著(P<0.05)。散养牛和圈养牛温氏支原体阳性率分别为42.4%(39/92)和16.3%(15/92),牛嗜血支原体待定种阳性率分别为22.1%(19/86)和6.9%(6/86)。散养牛温氏支原体阳性率和牛嗜血支原体待定种阳性率均显著高于圈养牛(P<0.05)。说明吉林省牛嗜血支原体普遍流行,但各地区流行种类和阳性率有明显差异,且阳性率随年龄的增加而升高,散养牛阳性率高于圈养牛。
In order to understand the prevalence of cattle Mycoplasma haemobos in Jilin Province,in the test,a total of 178 bovine blood samples were collected from seven regions in Jilin Province(Siping City,Liaoyuan City,Tonghua City,Baishan City,Shulan City,Songyuan City and Hunchun City)from July to September 2023.After extracting the blood DNA,the 16S rDNA gene of cattle Mycoplasma haemobos was amplified by PCR and sequenced,and the sequencing results of some positive samples of cattle Mycoplasma haemobos were randomly selected for the genetic evolution analysis.The chi-square test was also used to analyze the significance of differences in the positivity rates of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in blood samples from cattle of different regions,ages and breeding methods,respectively.The results showed that a total of 79 cattle Mycoplasma haemobos positive samples were detected from 178 blood samples.Among them,58 samples were positive for Mycoplasma wenyonii,with a positivity rate of 32.6%(58/178);21 samples were positive for Candidatus Mycoplasma haemobos,with a positivity rate of 11.8%(21/178);and there was no mixed infection.Mycoplasma wenyonii and cattle Mycoplasma haemobos to be defined were located on two major branches of the phylogenetic tree,respectively.Mycoplasma wenyonii Mw-1 was located in the same sub-branch with Henan strain Fengdu(FJ375309.1),with 100%nucleotide similarity and the closest relationship;Mycoplasma wenyonii Mw-2 was located in the same sub-branch with Mexican strain INIFAP01(KX171205.1),with 99.9%nucleotide similarity and the closest relationship;Candidatus Mycoplasma haemobos Cm-1 was located in the same sub-branch with Malaysian strain Clone1924712(KT985638.1),with 100%nucleotide similarity and closest relationship.The positivity rate of Mycoplasma wenyonii was the highest in Siping City(73.3%,14/19)and the lowest in Shulan City(5.3%,1/19);the positivity rate of Candidatus Mycoplasma haemobos was the highest in Hunchun City(25.0%,10/40),and the lowest in Siping and Liaoyuan City(0,0/19;0,0/20).The positive rates of Mycoplasma wenyonii were all significantly different between different regions(P<0.05);except for the positive rates of Candidatus Mycoplasma haemobos in Siping City and Liaoyuan City,which were not significantly different(P>0.05),the positive rates of Candidatus Mycoplasma haemobos in all other regions were significantly different(P<0.05).The positive rates of Mycoplasma wenyonii in<1 year old,≥1-<3 year old,and≥3 year old cattle were 0(0/6),25.0%(22/88),and 42.9%(36/84),respectively;and those of Candidatus Mycoplasma haemobos were 0(0/6),10.2%(9/88),and 14.2%(12/84),respectively,all of which increased with age.Significant differences(P<0.05)were found in both Mycoplasma wenyonii positivity and CandidatusMycoplasma haemobos positivity rates between cattle of different ages.The positive rates of Mycoplasma wenyonii in free-ranging and captive cattle were 42.4%(39/92)and 16.3%(15/92),respectively;the positive rates of Candidatus Mycoplasma haemobos were 22.1%(19/86)and 6.9%(6/86),respectively.The positive rates of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in free-ranging cattle were significantly higher than those in captive cattle(P<0.05).The results indicated that cattle Mycoplasma haemobos was generally prevalent in Jilin Province,but the prevalent species and positivity rate varied significantly among regions,and the positivity rate increased with age,and the positivity rate of free-ranging cattle was higher than that of captive cattle.
作者
杨孝龙
冯文勇
王贤炅
金春梅
于龙政
YANG Xiaolong;FENG Wenyong;WANG Xianjiong;JIN Chunmei;YU Longzheng(College of Agriculture,Yanbian University,Yanji 133002,China)
出处
《黑龙江畜牧兽医》
北大核心
2025年第7期83-88,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(32060807)。
关键词
嗜血支原体
吉林省
牛
温氏支原体
牛嗜血支原体待定种
Mycoplasma haemobos
Jilin Province
cattle
Mycoplasma wenyonii
Candidatus Mycoplasma haemobos
