摘要
胚胎干细胞(embryonic stem cells, ESCs)是一种来源于早期胚胎的多能干细胞,具有无限增殖、自我更新和多向分化的特性。无论在体外还是体内环境,ESCs都能被诱导分化为机体的大多数细胞类型,这些细胞通常来自于胚胎体外受精后的早期阶段。本文根据国内外ESCs体外培养的相关研究,对哺乳动物胚胎干细胞的生物学特性、获取、体外培养及分子标记进行综述,以期为哺乳动物ESCs长期培养和建系提供借鉴。
Embryonic stem cells(ESCs)are pluripotent stem cells derived from early embryos characterized by their ability to proliferate infinitely,self-renewal,and differentiate into multiple cell types.Both in vitro and in vivo,ESCs can be induced to differentiate into almost all cell types in the body.This paper provided a review of the biological characteristics of mammalian ESCs,their isolation,in vitro culture,and molecular markers,based on relevant research from home and abroad,aiming to provide reference for the long-term cultivation and establishment of mammalian ESCs.
作者
朱丽娜
安雪姣
王海锋
张东强
张瑞
徐振飞
李建烨
岳耀敬
ZHU Lina;AN Xuejiao;WANG Haifeng;ZHANG Dongqiang;ZHANG Rui;XU Zhenfei;LI Jianye;YUE Yaojing(Lanzhou Institute of Animal husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)
出处
《黑龙江畜牧兽医》
北大核心
2025年第7期16-24,共9页
Heilongjiang Animal Science And veterinary Medicine
基金
国家重点研发计划项目(2021YFD1300901)
甘肃省科技重大专项计划项目(22ZD6NA037)
甘肃省科技计划项目(21JR7RA033)
内蒙古自治区科技计划项目(2021GG320)。
关键词
胚胎干细胞
生物学特性
胚胎干细胞的获取
体外培养
分子标记
embryonic stem cells
biological characteristics
isolation of embryonic stem cells
in vitro culture
molecular markers
