摘要
链霉菌(Streptomyces)作为天然药物合成的重要模式菌,具有合成多种生物活性物质的潜力,有超过2/3的抗生素由链霉菌产生。然而,许多链霉菌的原始生产菌株存在着不易培养、生长缓慢、遗传操作困难等问题,这使得通过遗传改造的方式来提高目标化合物产量的策略受到了限制,同时本底产生的代谢物也会对目标天然产物的分离和提取产生一定的干扰。因此,开发生长快速、代谢背景清晰、遗传操作简单的链霉菌底盘细胞作为宿主,能解决天然药物产量低、生产成本高等问题。本文结合前期的研究工作,对链霉菌较小基因组的设计、构建及其在天然产物发掘和生产中的应用进行了综述,以期为利用链霉菌合成天然产物提供参考和借鉴。
The genus Streptomyces provides a wealth of natural products with diverse structures,accounting for more than two-thirds of natural antibiotics.However,the original Streptomyces producers of desired natural products are technically challenging due to their harsh cultivation conditions,slow growth rate and intricate genetic backgrounds.Meanwhile,the metabolites of Streptomyces can also cause interference with the extraction of the target natural products.Thus,there is an urgent need to develop an ideal Streptomyces chassis with characterization of clean metabolic background,fluent genetic manipulation,and higher success rate of biosynthetic gene cluster expressions,which can largely increase yield and reduce production costs of natural products.In this review,we summarize the recent development of the chassis in Streptomyces and its important roles in the excavation and production of natural products,with focus on its potential for discovery and synthesis of natural products.
作者
李慧
郭莉娟
周贺霞
李春阳
代佳琳
谌立祝
张锐
孙丰慧
Hui Li;Lijuan Guo;Hexia Zhou;Chunyang Li;Jialin Dai;Lizhu Shen;Rui Zhang;Fenghui Sun(School of Laboratory Medicine,Chengdu Medical College,Chengdu 610500,China)
出处
《遗传》
北大核心
2025年第7期756-767,共12页
Hereditas(Beijing)
基金
成都医学院校基金(编号:CYZYB24-07)
四川省动物源性食品兽药残留防控技术工程实验室开放基金(编号:24LHGCSYS1-04,24LHGCSYS1-06)
发育与再生四川省重点实验室开放基金(编号:24LHFYSZ1-48)资助。
关键词
链霉菌
较小基因组
天然产物
Streptomyces
minimal genome
natural products
