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香菇菌棒制备和菌丝控温培养关键技术 被引量:1

Key Technologies for the Preparation of Lentinula edodes Artificial Bed-Log and the Controlled-temperature Cultivation of Mycelium
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摘要 分析了不同接种时间段和菌丝培养条件对香菇菌棒成品率的影响;测定了接种后接种室和强冷室的杂菌数量;将接种后的菌棒至于24℃条件下分别培养0、4、8、12、16、20 d后,再转至菇棚进行常温培养,比较控温培养时间对香菇菌棒成品率和子实体产量的影响。结果表明,接种时间段对香菇菌棒成品率的影响不明显,但菌丝培养条件对菌棒成品率的影响显著。相比而言强冷室中的菌落数量更多,说明强冷室是制约接种环境的关键因素,可通过规划运行路线、控制人员走动等措施提高环境的洁净度。控温培养可以提高香菇菌棒的成品率和子实体产量,其中菌棒先控温培养16 d,再转运至菇棚进行常温培养为最优条件,该条件下香菇菌棒成品率和子实体产量均最高。 The effects of different inoculation periods and mycelial culture conditions on the completion rate of Lentinula edodes artificial bed-log were analyzed.The number of weed mould in the inoculation room and strong cold room was measured after inoculation.Cultivate the inoculated artificial bed-log at 24℃for 0,4,8,12,16,and 20 days respectively,and then transfer them to a mushroom shed for room-temperature cultivation.Compare the effects of temperature-controlled cultivation duration on the completion rate of L.edodes artificial bed-log and the yield of fruit bodies.The results showed that the inoculation periods had no significant effect on the completion rate of L.edodes artificial bed-log,but the mycelial culture conditions had a significant effect on the completion rate of L.edodes artificial bed-log.In comparison,there are more colonies in the strong cold room,indicating that it is a key factor restricting the inoculation environment.Measures such as planning operating routes and controlling personnel movement can be taken to improve the cleanliness of the environment.Temperature-controlled cultivation can improve the completion rate of L.edodes artificial bed-log and the yield of fruit bodies.The optimal condition is to cultivate the L.edodes artificial bed-log for 16 days at a controlled temperature,and then transfer the artificial bed-log to the mushroom shed for room-temperature cultivation.At this conditions,the completion rate of L.edodes artificial bed-logs and the yield of fruit bodies were the highest.
作者 胡建平 陈青 HU Jianping;CHEN Qing(Qingyuan Edible Fungi Industry Center(Qingyuan Edible Fungus Research Center),Qingyuan 323800,China;Zhejiang Engineering Research Center of Edible Mushroom Biotechnology,Qingyuan 323800,China;Zhejiang Key Laboratory of Biological Breeding and Exploitation of Edible and Medicinal Mushrooms,Qingyuan 323800,China;Agricultural Technology Extension Center of Zhejiang Province,Hangzhou 310020,China)
出处 《中国食用菌》 2025年第3期49-52,58,共5页 Edible Fungi of China
基金 浙江省农业重大技术协同推广计划(2023ZDXT09-4) 浙江省食用菌新品种选育重大科技专项(2021C02073-1)。
关键词 香菇 控温培养 菌棒 成品率 子实体产量 Lentinula edodes controlled-temperature cultivation artificial bed-log completion rate yield of fruit bodies
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