摘要
Current radiotherapy(RT)lacks the ability to accurately discriminate between tumor and healthy tissues,resulting in significant radiation-induced damage for patients.Therefore,there is an urgent need for precise RT techniques that can optimize tumor control while minimizing adverse effects on surrounding healthy tissues.In this study,we developed a nanodrug(AuNR@Peptide)composed of furin-responsive RVRR peptide-conjugated AuNRs,which integrates an activatable probe and a radiosensitizer into a single system for accurate tumor localization,enabling imageguided precision RT.Upon reaching the tumor site after intravenous administration,proteolytic cleavage of RVRR substrates on AuNR@Peptide by biomarker triggers aggregation of gold nanorods(AuNRs)into larger aggregates,leading to activation of near-infrared(NIR)-II photoacoustic(PA)signals to precisely localize the tumor and enhance tumor retention by preventing migration and backflow of AuNRs.This significantly amplifies radiosensitivity efficiency.The peak time point at which the NIR-II PA signal was observed at the tumor site after injection serves as a reference for initiating RT,demonstrating substantial improvement in tumor RT through investigations related to radiosensitization mechanisms.The integration of imaging and therapy in this study offers a promising image-guided therapeutic modality for tumors.
基金
supported by the Taishan Scholar Youth Expert Program in Shandong Province(Grant Number:tsqnz20230608)
grants from the Natural Science Foundation of Shandong Province(Grant Number:ZR2023QB045)
Scientific Research of Distinguished Professor from Qingdao University,China(Grant Numbers:DC2200000953,RZ2300002607,and RZ2400001462)
grant from Natural Science Foundation of Qingdao Municipality,Shandong Province,China(Grant Number:23-2-1-30-zyyd-jch).
