摘要
目的探讨前列腺癌组织中富含半胱氨酸肠蛋白2(CRIP2)表达与微小核糖核酸(miR)-362-5p/N-myc下游调节基因1(NDRG1)轴的相关性及对前列腺癌多西他赛(DTX)敏感性的影响。方法选取2021年3月至2023年10月南阳市中心医院收治的36例DTX敏感的前列腺癌术后化疗患者、36例DTX耐药的前列腺癌术后化疗患者,分别纳入DTX敏感组、DTX耐药组;均于术中留取患者前列腺癌组织、癌旁组织。比较两组患者前列腺癌组织CRIP2表达阳性率、miR-362-5p/NDRG1轴指标水平,采用点二列相关性分析癌组织CRIP2表达与miR-362-5p、NDRG1的相关性,采用多因素Logistic回归法分析癌组织CRIP2表达、miR-362-5p/NDRG1轴对前列腺癌DTX敏感性的影响,采用交互作用系数(γ)分析癌组织CRIP2、miR-362-5p/NDRG1轴的交互作用对前列腺癌DTX敏感性的影响,采用受试者工作特征曲线(ROC)分析癌组织CRIP2、miR-362-5p/NDRG1轴对前列腺癌DTX耐药的评估作用。结果DTX敏感组癌组织CRIP2表达阳性率为50.00%(18/36),低于DTX耐药组的75.00%(27/36),差异有统计学意义(P<0.05);DTX敏感组miR-362-5p表达量为0.74±0.09,明显低于DTX耐药组的0.98±0.07,DTX敏感组NDRG1蛋白表达量为0.58±0.11,明显高于DTX耐药组的0.29±0.04,差异均有统计学意义(P<0.05);相关性分析显示组织CRIP2表达与miR-362-5p表达量呈正相关(r=0.537),与NDRG1蛋白表达量呈负相关(r=-0.602),P<0.05;Logistic回归分析显示癌组织CRIP2表达、miR-362-5p、NDRG1均为前列腺癌患者DTX耐药的独立影响因素(P<0.05);癌组织CRIP2阳性与miR-362-5p高表达、癌组织CRIP2阳性与NDRG1蛋白低表达同时暴露时交互作用OR分别为31.167、25.333,γ分别为2.216、2.074,两两呈正向交互作用(P<0.05);ROC曲线结果显示,癌组织CRIP2、miR-362-5p/NDRG1轴联合评估前列腺癌DTX耐药的AUC最大,为0.945(95%CI:0.865~0.985,P<0.05)。结论癌组织CRIP2表达与miR-362-5p/NDRG1轴存在明显相关性,且均与前列腺癌DTX敏感性有关,检测癌组织CRIP2表达与miR-362-5p/NDRG1轴水平对评估前列腺癌DTX敏感性具有重要意义。
Objective To investigate the correlation between cysteine-rich intestinal protein 2(CRIP2)expres-sion and the microRNA(miR)-362-5p/N-myc downstream-regulated gene 1(NDRG1)axis in prostate cancer tissues,and their influence on docetaxel(DTX)sensitivity.Methods A total of 36 postoperative prostate cancer patients with DTX-sensitive chemotherapy and 36 with DTX-resistant chemotherapy treated at Nanyang Central Hospital from March 2021 to October 2023 were enrolled and assigned to DTX-sensitive and DTX-resistant groups,respectively.Prostate can-cer tissues and adjacent tissues were collected during surgery.The CRIP2-positive expression rate and miR-362-5p/NDRG1 axis levels were compared between groups.Point-biserial correlation analysis assessed the relationship between CRIP2 expression and miR-362-5p/NDRG1.Multivariate logistic regression was used to analyze the influence of CRIP2 and the miR-362-5p/NDRG1 axis on DTX sensitivity.Interaction coefficients(γ)was used to evaluate their com-bined effects,while receiver operating characteristic(ROC)curves was used to assess their predictive value for DTX re-sistance.Results The CRIP2-positive rate in the DTX-sensitive group(50.00%,18/36)was significantly lower than that in the resistant group(75.00%,27/36),P<0.05.miR-362-5p expression was lower,while NDRG1 protein was high-er in the sensitive group(both P<0.05):(0.74±0.09)vs(0.98±0.07),(0.58±0.11)vs(0.29±0.04).CRIP2 correlated posi-tively with miR-362-5p(r=0.537)and negatively with NDRG1(r=-0.602),P<0.05.Logistic regression identified CRIP2,miR-362-5p,and NDRG1 as independent factors influencing DTX resistance(P<0.05).Interaction analysis re-vealed synergistic effects between CRIP2 positivity and miR-362-5p overexpression(OR=31.167,γ=2.216)or NDRG1 underexpression(OR=25.333,γ=2.074),P<0.05.ROC analysis showed the combined model(CRIP2+miR-362-5p/NDRG1)had the highest AUC(0.945,95%CI:0.865-0.985)for predicting resistance.Conclusion CRIP2 expression correlates significantly with the miR-362-5p/NDRG1 axis,and both are associated with DTX sensitivity in prostate can-cer.Assessing these biomarkers may help evaluate therapeutic response.
作者
赵得堡
张钧硕
刘璐璐
孙星
刘越
屈中玉
ZHAO De-bao;ZHANG Jun-shuo;LIU Lu-lu;SUN Xing;LIU Yue;QU Zhong-yu(Department of Oncology,Nanyang Central Hospital,Nanyang 473000,Henan,CHINA)
出处
《海南医学》
2025年第13期1832-1837,共6页
Hainan Medical Journal
基金
2024年度河南省医学科技攻关计划联合共建项目(编号:LHGJ20240779)。
