摘要
目的探讨牙龈卟啉单胞菌(Pg)感染慢性牙周炎患者血清Toll样受体4(TLR4)、基质金属蛋白酶-3(MMP-3)、白细胞介素-1β(IL-1β)、外周血辅助性T细胞17/调节性T细胞(Th17/Treg)水平,及其对慢性牙周炎患者Pg感染的诊断价值。方法选取2022年1月-2024年1月于天津市口腔医院就诊的390例慢性牙周炎患者作为研究对象,根据Pg感染情况分为感染组(113例)和未感染组(277例),另外将感染组患者依据病情分为轻度组(31例)、中度组(45例)和重度组(37例)。比较感染组和未感染组及不同病情慢性牙周炎Pg感染患者血清TLR4、MMP-3、IL-1β、外周血Th17/Treg水平,并将感染组纳入阳性,未感染组纳入阴性,绘制受试者工作特征(ROC)曲线获取曲线下面积(AUC),分析血清TLR4、MMP-3、IL-1β、外周血Th17/Treg单一及联合检测对慢性牙周炎患者Pg感染的诊断价值。结果感染组血清TLR4、MMP-3、IL-1β及外周血Th17/Treg水平分别为(20.41±5.12)ng/ml、(6.12±2.03)μg/L、(10.24±2.19)μg/L及(0.32±0.10)高于未感染组(P<0.05)。重度组血清TLR4、MMP-3、IL-1β及外周血Th17/Treg水平分别为(22.58±6.10)ng/ml、(8.12±2.67)μg/L、(12.49±3.10)μg/L及(0.52±0.15)高于中度组[(14.78±3.44)ng/ml、(6.15±2.07)μg/L、(7.23±1.99)μg/L及(0.22±0.07)]和轻度组(P<0.05),中度组高于轻度组(P<0.05)。ROC分析结果显示,血清TLR4、MMP-3、IL-1β、外周血Th17/Treg联合检测诊断慢性牙周炎患者Pg感染的AUC为0.937,高于四者单一检测(P<0.05)。结论血清TLR4、MMP-3、IL-1β及外周血Th17/Treg在Pg感染的慢性牙周炎患者中呈高表达,四者可参与患者病情进展过程,且四者联合检测对慢性牙周炎患者Pg感染的诊断价值更高。
OBJECTIVE To investigate the serum toll-like receptor 4(TLR4),matrix metalloproteinase-3(MMP-3),in-terleukin-1β(IL-1β)and peripheral blood helper T-cell 17/regulatory T-cell(Th17/Treg)levels in patients with chronic periodontitis infected with porphyromonas gingivalis(Pg),as well as their diagnostic value of Pg infection in patients with chronic periodontitis.METHODS Totally 390 cases of patients with chronic periodontitis who at-tended Tianjin Stomatological Hospital from Jan.2022 to Jan.2024 were selected as the study subjects,and were divided into the infected group(113 cases)and the uninfected group(277 cases)according to Pg infection,and those patients in the infected group were further classified into the mild group(31 cases),the moderate group(45 cases),and the severe group(37 cases)according to the condition of the disease.Serum TLR4,MMP-3,IL-1β,and peripheral blood Th17/Treg levels were compared between the infected and uninfected groups and patients with different conditions of chronic periodontitis Pg infection,and the infected group was included in the positive and the uninfected group was included in the negative,and the receiver's operating characteristics(ROC)curves were plotted to obtain the area under the curve(AUC)to analyze the diagnostic value of single and combined de-tection of serum TLR4,MMP-3,and IL-1β,Peripheral blood Th17/Treg for Pg infection in patients with chronic periodontitis.RESULTS Serum TLR4,MMP-3,IL-1βand peripheral blood Th17/Treg levels in the infected group were(20.41±5.12)ng/ml,(6.12±2.03)μg/L,(10.24±2.19)μg/L and(0.32±0.10),higher than those in the uninfected group(P<0.05).Serum TLR4,MMP-3,IL-1βand peripheral blood Th17/Treg levels in the se-vere group were(22.58±6.10)ng/ml,(8.12±2.67)μg/L,(12.49±3.10)μg/L and(0.52±0.15),higher than those in the moderate group[(14.78±3.44)ng/ml,(6.15±2.07)μg/L,(7.23±1.99)μg/L and(0.22±0.07)]and mild group(P<0.05),which in the moderate group was higher than those in the mild group(P<0.05).The results of ROC analysis showed that the AUC of serum TLR4,MMP-3,IL-1β,and peripheral blood Th17/Treg combined test for diagnosing Pg infection in patients with chronic periodontitis was 0.937,which was higher than that of the four single tests(P<0.05).CONCLUSION Serum TLR4,MMP-3,IL-1βand peripheral blood Th17/Treg were highly expressed in chronic periodontitis patients infected with Pg,and all four indicators can participate in the progression of the disease,and the combined detection of these four indicators has a higher diagnostic value for Pg infection in patients with chronic periodontitis.
作者
郭庆杰
赵兴福
侯佳龙
韦欣
GUO Qingjie;ZHAO Xingfu;HOU Jialong;WEI Xin(Tianjin Stomatological Hospital,Tianjin 300041,China)
出处
《中华医院感染学杂志》
北大核心
2025年第11期1660-1664,共5页
Chinese Journal of Nosocomiology
基金
天津市科技发展计划基金资助项目(2022YX0054)。
