摘要
为筛选能够降解毛竹(Phyllostachys edulis)林经营中遗留的大量伐桩和鞭根中木质素的细菌,以解决木质素降解缓慢的问题。采集1龄、3龄毛竹篼根土和鞭根土,以苯胺蓝培养基为筛选培养基对根际木质素降解菌进行高通量分离培养;通过构建16S rRNA系统发育树鉴定细菌的种类,并通过定性和定量的方法筛选高效木质素降解细菌,结合木质素降解酶活性,解析其高效降解木质素的机制。结果表明:从毛竹根际共分离出80株具有木质素降解能力的细菌,其中,根际共有菌株粘质沙雷氏菌(Serratia marcescens,a26、b18)、嗜线虫沙雷氏菌(Serratia nematodiphila,b4、c11)和氧化木糖无色杆菌(Achromobacter xylosoxidans,b23、c10)的苯胺蓝脱色率最高,分别为69.14%、70.02%和68.79%。3种类型的根际中,总细菌的苯胺蓝脱色率无显著差异,但根际细菌种类存在特异性。在脱色率大于60%的菌株中,嗜线虫沙雷氏菌(c11)的木质素过氧化物酶和锰过氧化物酶活性最高,分别为19.14和40.69 U·L^(-1);马氏无色杆菌(Achromobacter marplatensis,b24)的锰过氧化物酶活性最高,达40.13 U·L^(-1);粘质沙雷氏菌(S.marcescens,a26)的漆酶活性最高,达143.89 U·L^(-1)。嗜线虫沙雷氏菌(c11)、粘质沙雷氏菌(a26)和马氏无色杆菌(b24)的木质素降解能力最强,且均为两种根际的共有菌株,在毛竹篼根和鞭根降解时具有一定的优势。
To screen for bacteria capable of degrading lignin in the numerous stumps and rhizome roots left from Phyllostachys edulis forest management,addressing the issue of slow lignin degradation.Soil around stump roots and rhizome roots of 1-year-old and 3-year-old P.edulis was collected.Aniline blue medium was used as the screening medium for high-throughput isolation and culture of rhizosphere lignin-degrading bacteria.Bacterial species were identified by constructing 16S rRNA phylogenetic trees.Highly efficient lignin-degrading bacteria were screened via qualitative and quantitative methods,and their efficient lignin degradation mechanisms were analyzed by combining lignin-degrading enzyme activities.The results showed that:A total of 80 lignin-degrading bacterial strains were isolated from the P.edulis rhizosphere.Among them,the rhizosphere shared strains Serratia marcescens(a26,b18),Serratia nematodiphila(b4,c11),and Achromobacter xylosoxidans(b23,c10)had the highest aniline blue decolorization rates,reaching 69.14%,70.02%,and 68.79%,respectively.There was no significant difference in the aniline blue decolorization rates of total bacteria among the three types of rhizosphere,but specific bacterial species existed in the rhizosphere.Among the strains with a decolorization rate greater than 60%,S.nematodiphila(c11)had the highest activities of lignin peroxidase and manganese peroxidase,which were 19.14 and 40.69 U·L^(-1),respectively.Achromobacter marplatensis(b24)had the highest manganese peroxidase activity,reaching 40.13 U·L^(-1).S.marcescens(a26)had the highest laccase activity,reaching 143.89 U·L^(-1).S.nematodiphila(c11),S.marcescens(a26),and A.marplatensis(b24)had the strongest lignin degradation capabilities,and all were common strains in the two types of rhizosphere,showing certain advantages in degrading P.edulis stump roots and rhizome roots.
作者
江明君
曹婷婷
蒋迎楹
曾文婷
江依婷
杨显银
宋新章
施曼
Jiang Mingjun;Cao Tingting;Jiang Yingying;Zeng Wenting;Jiang Yiting;Yang Xianyin;Song Xinzhang;Shi Man(State Key Laboratory of Subtropical Silviculture(Zhejiang A&F University),Hangzhou 311300,P.R.China)
出处
《东北林业大学学报》
北大核心
2025年第9期48-54,共7页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(32471975、32125027、31930075)
浙江省大学生科技活动计划暨新苗人才计划项目(2024R412A003)。
关键词
木质素降解菌
高通量筛选
分离培养
木质素过氧化物酶
毛竹
Lignin-degrading bacteria
High-throughput screening
Isolation and culture
Lignin peroxidase
Phyllostachys edulis
