摘要
Aims:Shangxia Liangji formula(SXLJF)is a traditional Chinese medicine formula used clinically for the treatment of insomnia.However,the exact mechanism of SXLJF remains unclear.Methods and Results:A mouse insomnia model was established by intra-peritoneal injection of p-chlorophenylalanine.Control,model,low/medium/high-dose SXLJF(9.26,18.72,and 37.44 g/kg/day),and diazepam(2 mg/kg)groups were treated with designated reagents for 7 days.Behavioral changes were assessed via open field and pentobarbital-induced sleep tests.Hippo-campal histopathology was evaluated using HE,Nissl,and TUNEL staining.Levels of serotonin(5-HT),5-HIAA,dopamine(DA),norepinephrine(NE),IL-1β,IL-6,and TNF-αwere measured using ELISA.RT-qPCR and immunoflu-orescence were used to analyze glial fibrillary acidic protein(GFAP),neuronal nuclei(NeuN),and brain-derived neurotrophic factor(BDNF)expression.Behavioral tests showed that SXLJF reduced mouse total distance traveled,average velocity,central distance,and sleep latency but increased sleep duration.It also attenuated expression of IL-1β,IL-6,and TNF-α,modulated levels of the monoamine neurotransmitters 5-HT,5-HIAA,NE,and DA,upre-gulated NeuN and BDNF expression,downregulated GFAP expression,inhibited astrocyte activation,and mitigated neuronal apoptosis.Conclusion:The mechanism underlying SXLJF’s anti-insomnia effect may be related to the regulation of monoamine neurotransmitters,inhibition of astro-cyte activation and inflammatory factors,and suppression of neuronal apoptosis.
基金
National Key Clinical Specialty Scientific Research Project,Grant/Award Number:Z2023161。
