摘要
为探明仿刺参应对高温胁迫的调控机制,采用Illumina HiSeq技术对高温组(30℃)和对照组(24℃)仿刺参幼参[(5.0±0.6)g]体壁样本进行转录组测序分析。高温组和对照组共产生了2920个差异表达基因,包括1342个上调表达基因和1578个下调表达基因。差异表达基因的KEGG信号通路分析表明,其显著富集在核糖体、内质网蛋白加工、病原互作、糖酵解和氧化磷酸化等通路中。对上下调表达基因在不同信号通路分布的研究发现,仿刺参受到热胁迫后体内信号转导活动增加,蛋白翻译活动减少,而对已有蛋白的折叠加工活动增加,整体的能量代谢下降。实时荧光定量PCR检测结果显示,5个基因在对照组和高温组的表达量相关系数R为0.87,表明转录组测序结果可靠。热休克蛋白在热胁迫响应过程中发挥重要作用,HSP90β和HSP70A/B2基因的高表达促进了蛋白的正确折叠,增强了耐热性。仿刺参幼参在应对高温胁迫时对已有蛋白的折叠加工活动增加,减少了蛋白的翻译和能量消耗,这是机体维护体内稳态、保护自身免受损伤的一种策略。对热胁迫相关基因SNP位点的分析,有助于分子标记的开发和选育耐高温苗种。
In order to explore the regulatory mechanism of juvenile sea cucumber Apostichopus japonicus in response to high temperature stress,transcriptome sequencing analysis was conducted in the body wall in the sea cucumber with body weight of(5.0±0.6)g exposed to water temperature of 30℃raising from 24℃at a rate of 1℃/h(high temperature group)and of 24℃(control group).Transcriptome comparison revealed that there were 2920 differentially expressed genes(DEGs)in the body wall o fthe juvenile between the high-temperature group and the control group,including 1342 up-regulated expression genes and 1578 down-regulated expression genes.KEGG analysis showed that the DEGs were found to be significantly enriched in ribosome,protein processing in endoplasmic reticulum,pathogen interaction,glycolysis and oxidative phosphorylation pathways.The distribution of up and down regulated genes in signaling pathways showed that the signal transduction activity was increased,with decrease in the protein translation activity,increase in the folding and processing activity of existing proteins,and decrease in overall energy metabolism in the juvenile exposed to high-temperature stress.The expression levels of five genes were detected in control group and high-temperature group by quantitative real time PCR,with correlation coefficient R of 0.87,indicating that the results of transcriptome sequencing were reliable.In response to high-temperature stress,the high expression of HSP90βand HSP70A/B2 genes promoted the correct folding of protein and enhance the heat resistance,via increase in protein folding and processing.Protein translation and energy consumption were shown to be decreased,which is a strategy to maintain homeostasis and protect from injury in juvenile.Screening single nucleotide polymorphisms(SNP)on mRNAs associated with high-temperature stress is beneficial for the development of molecular markers and breeding of new heat-resistant varieties in A.japonicus.
作者
孙红娟
蒋经伟
宋钢
陈仲
关晓燕
高杉
王摆
姜苹哲
李佩佩
岳冬梅
肖瑶
潘永嘉
姜冰
董颖
SUN Hongjuan;JIANG Jingwei;SONG Gang;CHEN Zhong;GUAN Xiaoyan;GAO Shan;WANG Bai;JIANG Pingzhe;LI Peipei;YUE Dongmei;XIAO Yao;PAN Yongjia;JIANG Bing;DONG Ying(Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource,Ministry of Agriculture and Rural Affairs,Liaoning Key Laboratory of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;Longhaixingang Science and Technology Co.,Itd.,Jinzhou 121209,China)
出处
《水产科学》
北大核心
2025年第4期553-561,共9页
Fisheries Science
基金
辽宁省科技计划项目(2023JH1/10200007,2022JH2/101300287)
辽宁省“兴辽英才”计划项目(XLYC2203191)
大连市科技人才创新支持计划项目(2023RJ007,2022RQ093)
大连市重点科技研发计划项目(2022YF16SN068)
辽宁省农业科学院院长基金资助项目(2023QN2419).
关键词
仿刺参
高温
转录组测序
差异表达基因
热休克蛋白
Apostichopus japonicus
high temperature
transcriptome sequencing
differentially expressed gene
heat shock protein
