摘要
基于成像的三维空间转录组学技术通过整合空间转录组学、成像技术与组织透明化技术,突破传统空间转录组学二维空间的局限性,实现转录本的三维空间原位解析.本文系统综述以基于滚环扩增、杂交链式反应及多重荧光原位杂交为代表的三类三维空间转录组学技术发展,总结当前这些技术突破二维成像的局限性所进行的相关技术优化细节,以及比较现有的基于成像的三维空间转录组学技术的各种性能,并对该领域未来发展提出思考,旨在为未来三维空间转录组学技术的开发提供见解.
Imaging-based 3D spatial transcriptomics integrates spatial transcriptomics imaging with advanced tissue-clearing methods to transcend the inherent limitations of conventional two-dimensional approaches, enabling precise three-dimensional in situ mapping of transcripts. In this paper, we systematically review advancements in 3D spatial transcriptomics technologies grounded in rolling circle amplification, hybridization chain reaction, and multiplexed fluorescence in situ hybridization. We further summarize technical optimizations addressing challenges in transitioning from 2D to 3D analysis, compare the performance of existing imaging-based three-dimensional spatial transcriptomics platforms, and provide insights into future developments in this field, ultimately aiming to offer guidance for the development of 3D spatial transcriptomics technologies.
作者
王浩贤
江伏青
邹商杰
彭广敦
WANG HaoXian;JIANG FuQing;ZOU ShangJie(PENG GuangDun11Center for Cell Lineage Technology and Engineering,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou510530,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《中国科学:生命科学》
北大核心
2025年第6期1149-1162,共14页
Scientia Sinica(Vitae)
基金
广东省基础与应用基础研究基金(批准号:2019B151502054,2022A1515110942)
广州国家实验室专项(批准号:GZNL2023A03005)
中国科学院广州生物医药与健康研究院基础研究项目(批准号:G1BHBRP23-01)资助。
