摘要
Sox2是一种胚胎干细胞抗原,对早期胚胎发育、组织稳态和免疫调节都至关重要。在本研究中,从淡水双壳动物背角无齿蚌体内克隆出一个完整的Sox2 cDNA序列,并命名为AwSox2。通过苏木精和伊红(H&E)染色分析双酚A(BPA)处理后的睾丸组织变化。使用实时定量PCR测定了BPA、脂多糖(LPS)和聚肌胞苷酸(Poly I:C)激发条件下的AwSox2表达水平。AwSox2的全长cDNA含有一个由927个核苷酸组成的可读框,具有Sox2家族的典型结构特征。在BPA处理组中观察到明显的精子细胞变性和不规则排列,以及聚集死亡和缠结精子细胞。BPA处理可导致背角无齿蚌睾丸中的AwSox2表达出现剂量依赖性的上调。此外,与对照组相比,LPS和Poly I:C(免疫激发剂)处理显著诱导肝胰脏、鳃和血细胞中AwSox2的表达。这些结果表明,AwSox2上调与BPA处理导致的精原干细胞凋亡以及双壳动物背角无齿蚌针对LPS和Poly I:C激发的免疫防御能力增强密切相关,从而提高对BPA对环境污染的重视。
Sox2 is an embryonal stem cell antigen essential for early embryonic development,tissue homeostasis and immune regulation.In the current study,one complete Sox2 cDNA sequence was cloned from freshwater bivalve Anodonta woodiana and named AwSox2.Histological changes of testis derived from BPA treatment were analyzed by hematoxylin and eosin(H&E)staining.Expressions of AwSox2 derived from bisphenol A(BPA),lipopolysaccharide(LPS)and polyinosinic:polycytidylic(Poly I:C)challenge were measured by quantitative real-time PCR,respectively.The full-length cDNA of AwSox2 contained an open reading frame of 927 nucleotides bearing the typical structural features of Sox2 family.Obvious degeneration and irregular arrangement of spermatids,clotted dead and intertwined spermatids were observed in BPA treated groups.Administration of BPA could result in a dose-dependent up-regulation of AwSox2 expression in the testis of bivalve A.woodiana.In addition,expression of AwSox2 was significantly induced by LPS and Poly I:C treatment in the hepatopancreas,gill and hemocytes compared with that of control group,respectively.These results indicate that up-regulations of AwSox2 are closely related with apoptosis of spermatogonial stem cells derived from BPA treatment as well as enhancement of immune defense against LPS and Poly I:C challenge in the bivalve A.woodiana,so as to raise our awareness of the environmental pollution caused by BPA.
作者
薛士鹏
连云峰
王奇正
夏西超
XUE Shipeng;LIAN Yunfeng;WANG Qizheng;XIA Xichao(Nanyang Medical University,Nanyang 473061,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)
出处
《生态毒理学报》
北大核心
2025年第3期287-298,共12页
Asian Journal of Ecotoxicology
基金
河南省自然科学基金项目(252300420142)
河南省科技攻关项目(212102310845)。
