摘要
植物钠氢反转运蛋白(NHX,Na^(+)/H^(+)antiporter)在植物钠钾离子平衡、细胞pH值调节中起重要的作用。为探究黑麦耐盐性与NHX的关系,通过生物信息学流程对黑麦的NHX基因进行鉴定与分析,结合RT-qPCR技术对ScNHXs在盐胁迫下的表达模式进行检测,为探究NHX基因在黑麦中的潜在功能以及黑麦耐盐基因挖掘等研究提供参考信息。共鉴定获得10个黑麦NHX基因家族成员(ScNHX1~ScNHX10),系统进化树分析表明,其可分为Vac和Endo 2个亚家族,分别包含4,6个基因。其编码蛋白理化性质分析表明,分子质量为27.92~59.72 ku,氨基酸数目为253~546 aa,等电点为5.17~8.81,大多数蛋白属于酸性蛋白,均未预测到信号肽存在但均具有跨膜结构,亚细胞定位预测ScNHXs定位于质膜和液泡中。空间结构预测显示,其二级结构主要由α-螺旋和无规则卷曲构成。基因结构和motif分析发现,ScNHXs的外显子数为13~24,且均具有保守的Na^(+)/H^(+)交换结构域。此外,顺式作用元件分析表明,ScNHXs启动子区域中均发现众多与激素响应以及非生物胁迫响应等生物过程相关的元件。黑麦转录组数据分析发现,在黑麦的不同组织中ScNHXs表达模式存在明显差异。RT-qPCR分析表明,ScNHXs对不同浓度NaCl胁迫有不同程度的响应,并且在较长时间内能够持续响应盐胁迫。综上所述,ScNHXs可能参与黑麦对盐胁迫的生物调节相关过程。
Plant sodium-hydrogen antiporter(NHX,Na^(+)/H^(+)antiporter)plays a crucial role in plant sodium and potassium ion balance and cellular pH regulation.In order to investigate the relationship between salt tolerance and ScNHXs,it was conducted to identify and analyze the ScNHXs by bioinformatics process,and to examine the expression pattern of ScNHXs under salt stress by RT-qPCR,which can provide the reference information for the investigation of the potential functions of ScNHXs as well as the mining of salt tolerance genes in rye.A total of 10 rye NHX gene family members(ScNHX1—ScNHX10)were identified,and the phylogenetic tree analysis showed that they could be divided into two subfamilies,Vac and Endo,containing four and six genes,respectively.Physicochemical property analysis of the encoded proteins showed that most of the molecular weight ranged from 27.92 to 59.72 ku,the number of amino acids from 253 to 546 aa,and the isoelectric point between 5.17 and 8.81,with most of proteins being classified as acidic proteins.Signal peptide prediction indicated the absence of signal peptides in the members,and transmembrane structure analysis revealed that all members possessed transmembrane structures.The subcellular localization prediction indicated that ScNHXs were located in the plasma membrane and vesicles.Spatial structure prediction showed that their secondary structures mainly consisted ofα-helices and irregular convolutions.Gene structure and motif analyses revealed that the number of exons of the ScNHXs varied from 13 to 24,and all of them possessed a conserved Na^(+)/H^(+)exchange structural domain.In addition,cis-acting element analysis revealed that numerous elements related to hormone response and abiotic stresses were found in the promoter region of ScNHXs.Analysis of rye transcriptome data revealed significant differences in the expression patterns of ScNHXs in different tissues of rye.RT-qPCR analysis showed that ScNHXs responded differently to different concentrations of NaCl stress,and were able to persistently respond to salt stress over a long period of time.In summary,ScNHXs may be involved in the biological regulation during salt stress in rye.
作者
王昭懿
崔原瑗
韩梦荞
刘正文
邓习
豆飞飞
任毓昭
刘彩霞
刘凤楼
王掌军
孙洋洋
任民
李清峰
WANG Zhaoyi;CUI Yuanyuan;HAN Mengqiao;LIU Zhengwen;DENG Xi;DOU Feifei;REN Yuzhao;LIU Caixia;LIU Fenglou;WANG Zhangjun;SUN Yangyang;REN Min;LI Qingfeng(School of Agriculture,Ningxia University,Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,China;Institute of Tobacco Research,Chinese Academy Agricultural Sciences,Qingdao 266000,China)
出处
《华北农学报》
北大核心
2025年第3期9-18,共10页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(32260467)
中国烟草总公司重大科技项目(110202201002(JY-02))
宁夏回族自治区科技重大专项(2022BSB03109)。
