摘要
目的建立不同产地喜树果中9个成分含量同步检测方法,筛选影响其质量的差异标志物,并对其进行质量评价。方法对7省18个批次喜树果样品进行回流提取,提取物采用高效液相色谱法检测;采用正交偏最小二乘判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)和加权逼近理想解排序(Technique for order preference by similarity to an ideal solution,TOPSIS)法建立喜树果质量优劣评价模型,对其质量差异性进行综合评价。结果3,4′-O-二甲基鞣花酸、丁香酸、10-羟基喜树碱、喜树碱、10-甲氧基喜树碱、三叶豆苷、短小蛇根草苷、金丝桃苷和喜果苷分别在0.51-12.75、0.23-5.75、3.21-80.25、4.45-111.25、1.88-47.00、0.41-10.25、2.05-51.25、0.34-8.50和7.95-198.75μg·mL^(-1)范围内线性关系良好(r>0.999),平均加样回收率96.95%-100.06%(RSD<2.0%);18批样品聚为3类;喜果苷、10-羟基喜树碱、喜树碱、10-甲氧基喜树碱和短小蛇根草苷可能是影响喜树果产品质量主要潜在标志物;加权TOPSIS法分析结果显示18批喜树果质量评价贴近度(Jb)在0.1090-0.7385,其中S14最大(0.7385)。结论建立了同时测定喜树果中9种成分含量的方法,操作简便、结果准确;采用OPLS-DA及加权TOPSIS法进行客观全面评价,可用于喜树果质量差异性评价。
Objective To screen the differential markers affecting their quality,and sort their quality,a method for simultaneous detection of 9 components in Camptothecae Fructus from different producing areas was established.Methods Reflux extraction was performed on 18 batches of Camptothecae Fructus from 7 provinces,and the extracts were detected by HPLC.A orthogonal partial least squares discriminant analysis and weighted TOPSIS method were used to establish a quality evaluation model for Camptothecae Fructus,and the quality differences were comprehensively evaluated.Results When the 9 components were in the 0.51-12.75,0.23-5.75,3.21-80.25,4.45-111.25,1.88-47.00,0.41-10.25,2.05-51.25,0.34-8.50 and 7.95-198.75μg·mL^(-1),their linear relationships were good(r>0.999).The average recovery were 96.95%-100.06%with the RSDs<2.0%.18 batches of samples were clustered into 3 categories.Vincosamide,10-hydroxycamptothecin,camptothecin 10-methoxycamptothecin and pumiloside may be the main potential markers affecting the quality of Camptothecae Fructus.The analysis results of the weighted TOPSIS method revealed that the closeness(Jb)for evaluating the quality of 18 batches of Camptothecae Fructus ranged from 0.1090 to 0.7385,with S14 achieving the highest value of 0.7385.Conclusion A method for simultaneous determination of 9 components in Camptothecae Fructus was established,which is simple and accurate.OPLS-DA and weighted TOPSIS method can be used to evaluate the quality difference of Camptothecae Fructus.
作者
李思阳
李更东
闫宇辉
LI Siyang;LI Gengdong;YAN Yuhui(School of Pharmacy,Jiangsu Food and Pharmaceutical Science College,Huai'an 223003,China;School of Life Science,Northeast Forestry University,Harbin 150040,China)
出处
《世界科学技术-中医药现代化》
北大核心
2025年第5期1376-1385,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
江苏省教育厅江苏省高校自然科学研究面上项目(17KJB360002):基于PepT1喜树碱拟肽化合物合成及硅质体制剂研究,负责人:李思阳
江苏高校“青蓝工程”项目(苏教师函[2022]51号):江苏省教育厅2023年江苏高校“青蓝工程”优秀青年骨干教师培养,负责人:李思阳。
